FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_A23MVY2LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23MVY2LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33914
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC76

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGG2182264.34510821489651TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTAGG796623.488824674175856TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGGG4511.3298342867252462TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGT3260.96125493896326TruSeq Adapter, Index 3 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGGGGG3000.8845904346287669TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTAGT2600.7666450433449313TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTAGGG1290.3803738868903698TruSeq Adapter, Index 3 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTAGGGGG850.2506339564781506TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATGTGGG740.21819897387509585TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCGGGG700.2064044347467123TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCGAGG590.1739694521436575TruSeq Adapter, Index 3 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGG510.15038037388689038TruSeq Adapter, Index 4 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATGTAGG490.1444831043226986TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATTTGGG470.1385858347585068TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGGG460.13563719997641094TruSeq Adapter, Index 4 (96% over 31bp)
GTCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGGG390.1149967565017397TruSeq Adapter, Index 4 (97% over 35bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGGG350.10320221737335615TruSeq Adapter, Index 3 (96% over 32bp)
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGTGTACAATCTGGG350.10320221737335615TruSeq Adapter, Index 4 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)