Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 33914 total bases: 5121014 Q20 bases: 4370446(85.3434%) Q30 bases: 3212915(62.7398%) Read2 before filtering: total reads: 33914 total bases: 5121014 Q20 bases: 4902644(95.7358%) Q30 bases: 3915386(76.4572%) Read1 after filtering: total reads: 841 total bases: 90847 Q20 bases: 85830(94.4775%) Q30 bases: 77117(84.8867%) Read2 after filtering: total reads: 841 total bases: 92083 Q20 bases: 90629(98.421%) Q30 bases: 84826(92.1191%) Filtering result: reads passed filter: 1682 reads failed due to low quality: 2204 reads failed due to too many N: 0 reads failed due to too short: 63942 reads with adapter trimmed: 33982 bases trimmed due to adapters: 4907762 Duplication rate: 44.029% Insert size peak (evaluated by paired-end reads): 35 JSON report: NTC_0001_0001_A23MVY2LT4_1.fastp.json HTML report: NTC_0001_0001_A23MVY2LT4_1.fastp.html fastp --in1 NTC_0001_0001_A23MVY2LT4_1_1.fastq.gz --in2 NTC_0001_0001_A23MVY2LT4_1_2.fastq.gz --out1 NTC_0001_0001_A23MVY2LT4_1_1.fastp.fastq.gz --out2 NTC_0001_0001_A23MVY2LT4_1_2.fastp.fastq.gz --json NTC_0001_0001_A23MVY2LT4_1.fastp.json --html NTC_0001_0001_A23MVY2LT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 1 seconds