Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 61416934 total bases: 9273957034 Q20 bases: 9015139068(97.2092%) Q30 bases: 8663112316(93.4133%) Read2 before filtering: total reads: 61416934 total bases: 9273957034 Q20 bases: 9061539082(97.7095%) Q30 bases: 8642092460(93.1867%) Read1 after filtering: total reads: 60075557 total bases: 7060779611 Q20 bases: 7029111299(99.5515%) Q30 bases: 6885230325(97.5137%) Read2 after filtering: total reads: 60075557 total bases: 7074568353 Q20 bases: 7030693243(99.3798%) Q30 bases: 6874540040(97.1726%) Filtering result: reads passed filter: 120151114 reads failed due to low quality: 1942802 reads failed due to too many N: 21470 reads failed due to too short: 718482 reads with adapter trimmed: 95360165 bases trimmed due to adapters: 4057633694 Duplication rate: 53.2857% Insert size peak (evaluated by paired-end reads): 108 JSON report: 659_dcz-T1-TRNA-1_A23MVY2LT4_2.fastp.json HTML report: 659_dcz-T1-TRNA-1_A23MVY2LT4_2.fastp.html fastp --in1 659_dcz-T1-TRNA-1_A23MVY2LT4_2_1.fastq.gz --in2 659_dcz-T1-TRNA-1_A23MVY2LT4_2_2.fastq.gz --out1 659_dcz-T1-TRNA-1_A23MVY2LT4_2_1.fastp.fastq.gz --out2 659_dcz-T1-TRNA-1_A23MVY2LT4_2_2.fastp.fastq.gz --json 659_dcz-T1-TRNA-1_A23MVY2LT4_2.fastp.json --html 659_dcz-T1-TRNA-1_A23MVY2LT4_2.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 170 seconds