[W::hts_idx_load3] The index file is older than the data file: 659_bQZ-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16561762) splicing reads. 282890 splicing junctions. 191594 known splicing junctions. 91296 novel splicing junctions.
sampling 10% (33123525) splicing reads. 348476 splicing junctions. 204365 known splicing junctions. 144111 novel splicing junctions.
sampling 15% (49685287) splicing reads. 393769 splicing junctions. 210928 known splicing junctions. 182841 novel splicing junctions.
sampling 20% (66247050) splicing reads. 428527 splicing junctions. 215158 known splicing junctions. 213369 novel splicing junctions.
sampling 25% (82808813) splicing reads. 456249 splicing junctions. 218174 known splicing junctions. 238075 novel splicing junctions.
sampling 30% (99370575) splicing reads. 478916 splicing junctions. 220480 known splicing junctions. 258436 novel splicing junctions.
sampling 35% (115932338) splicing reads. 498057 splicing junctions. 222204 known splicing junctions. 275853 novel splicing junctions.
sampling 40% (132494101) splicing reads. 514281 splicing junctions. 223652 known splicing junctions. 290629 novel splicing junctions.
sampling 45% (149055863) splicing reads. 528060 splicing junctions. 224828 known splicing junctions. 303232 novel splicing junctions.
sampling 50% (165617626) splicing reads. 540022 splicing junctions. 225803 known splicing junctions. 314219 novel splicing junctions.
sampling 55% (182179389) splicing reads. 550329 splicing junctions. 226652 known splicing junctions. 323677 novel splicing junctions.
sampling 60% (198741151) splicing reads. 559451 splicing junctions. 227317 known splicing junctions. 332134 novel splicing junctions.
sampling 65% (215302914) splicing reads. 567162 splicing junctions. 227870 known splicing junctions. 339292 novel splicing junctions.
sampling 70% (231864677) splicing reads. 573998 splicing junctions. 228392 known splicing junctions. 345606 novel splicing junctions.
sampling 75% (248426439) splicing reads. 580008 splicing junctions. 228829 known splicing junctions. 351179 novel splicing junctions.
sampling 80% (264988202) splicing reads. 585129 splicing junctions. 229188 known splicing junctions. 355941 novel splicing junctions.
sampling 85% (281549965) splicing reads. 589544 splicing junctions. 229507 known splicing junctions. 360037 novel splicing junctions.
sampling 90% (298111727) splicing reads. 593234 splicing junctions. 229783 known splicing junctions. 363451 novel splicing junctions.
sampling 95% (314673490) splicing reads. 596280 splicing junctions. 229987 known splicing junctions. 366293 novel splicing junctions.
sampling 100% (331235253) splicing reads. 598848 splicing junctions. 230160 known splicing junctions. 368688 novel splicing junctions.