#!/bin/bash -euo pipefail python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \ --SJ_tab_file PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.SJ.out.tab \ --chimJ_file PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.Chimeric.out.junction \ --bam_file PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam \ --output_prefix PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1 \ --ctat_genome_lib ctat_genome_lib_build_dir \ --vis --sample_name PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1 # Sort output files to ensure consistent ordering across runs while preserving headers if [ -f PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns ]; then # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron) head -n 1 PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns > PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns.tmp tail -n +2 PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns.tmp mv PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns.tmp PX_FFPE_pan_TRNA_nat_03_A23MVY2LT4_1.cancer.introns fi cat <<-END_VERSIONS > versions.yml "NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS": ctat-splicing: 0.0.3 END_VERSIONS