[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (20059359) splicing reads. 272202 splicing junctions. 183694 known splicing junctions. 88508 novel splicing junctions.
sampling 10% (40118718) splicing reads. 336987 splicing junctions. 196321 known splicing junctions. 140666 novel splicing junctions.
sampling 15% (60178077) splicing reads. 381923 splicing junctions. 203021 known splicing junctions. 178902 novel splicing junctions.
sampling 20% (80237437) splicing reads. 416646 splicing junctions. 207354 known splicing junctions. 209292 novel splicing junctions.
sampling 25% (100296796) splicing reads. 444150 splicing junctions. 210359 known splicing junctions. 233791 novel splicing junctions.
sampling 30% (120356155) splicing reads. 466596 splicing junctions. 212636 known splicing junctions. 253960 novel splicing junctions.
sampling 35% (140415514) splicing reads. 485538 splicing junctions. 214426 known splicing junctions. 271112 novel splicing junctions.
sampling 40% (160474874) splicing reads. 501372 splicing junctions. 215861 known splicing junctions. 285511 novel splicing junctions.
sampling 45% (180534233) splicing reads. 514795 splicing junctions. 217065 known splicing junctions. 297730 novel splicing junctions.
sampling 50% (200593592) splicing reads. 526708 splicing junctions. 218057 known splicing junctions. 308651 novel splicing junctions.
sampling 55% (220652951) splicing reads. 537075 splicing junctions. 218830 known splicing junctions. 318245 novel splicing junctions.
sampling 60% (240712311) splicing reads. 546163 splicing junctions. 219552 known splicing junctions. 326611 novel splicing junctions.
sampling 65% (260771670) splicing reads. 554141 splicing junctions. 220156 known splicing junctions. 333985 novel splicing junctions.
sampling 70% (280831029) splicing reads. 561016 splicing junctions. 220666 known splicing junctions. 340350 novel splicing junctions.
sampling 75% (300890388) splicing reads. 567031 splicing junctions. 221084 known splicing junctions. 345947 novel splicing junctions.
sampling 80% (320949748) splicing reads. 572179 splicing junctions. 221455 known splicing junctions. 350724 novel splicing junctions.
sampling 85% (341009107) splicing reads. 576654 splicing junctions. 221787 known splicing junctions. 354867 novel splicing junctions.
sampling 90% (361068466) splicing reads. 580421 splicing junctions. 222052 known splicing junctions. 358369 novel splicing junctions.
sampling 95% (381127825) splicing reads. 583508 splicing junctions. 222268 known splicing junctions. 361240 novel splicing junctions.
sampling 100% (401187185) splicing reads. 586111 splicing junctions. 222476 known splicing junctions. 363635 novel splicing junctions.