[W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18859679) splicing reads. 290651 splicing junctions. 191572 known splicing junctions. 99079 novel splicing junctions.
sampling 10% (37719358) splicing reads. 366418 splicing junctions. 204741 known splicing junctions. 161677 novel splicing junctions.
sampling 15% (56579038) splicing reads. 422191 splicing junctions. 211830 known splicing junctions. 210361 novel splicing junctions.
sampling 20% (75438717) splicing reads. 467223 splicing junctions. 216450 known splicing junctions. 250773 novel splicing junctions.
sampling 25% (94298396) splicing reads. 504817 splicing junctions. 219888 known splicing junctions. 284929 novel splicing junctions.
sampling 30% (113158076) splicing reads. 536394 splicing junctions. 222515 known splicing junctions. 313879 novel splicing junctions.
sampling 35% (132017755) splicing reads. 563979 splicing junctions. 224565 known splicing junctions. 339414 novel splicing junctions.
sampling 40% (150877434) splicing reads. 588043 splicing junctions. 226196 known splicing junctions. 361847 novel splicing junctions.
sampling 45% (169737114) splicing reads. 609024 splicing junctions. 227575 known splicing junctions. 381449 novel splicing junctions.
sampling 50% (188596793) splicing reads. 627412 splicing junctions. 228765 known splicing junctions. 398647 novel splicing junctions.
sampling 55% (207456472) splicing reads. 644039 splicing junctions. 229778 known splicing junctions. 414261 novel splicing junctions.
sampling 60% (226316152) splicing reads. 658644 splicing junctions. 230675 known splicing junctions. 427969 novel splicing junctions.
sampling 65% (245175831) splicing reads. 671420 splicing junctions. 231403 known splicing junctions. 440017 novel splicing junctions.
sampling 70% (264035510) splicing reads. 682719 splicing junctions. 232069 known splicing junctions. 450650 novel splicing junctions.
sampling 75% (282895190) splicing reads. 692781 splicing junctions. 232653 known splicing junctions. 460128 novel splicing junctions.
sampling 80% (301754869) splicing reads. 701506 splicing junctions. 233098 known splicing junctions. 468408 novel splicing junctions.
sampling 85% (320614548) splicing reads. 709071 splicing junctions. 233531 known splicing junctions. 475540 novel splicing junctions.
sampling 90% (339474228) splicing reads. 715547 splicing junctions. 233858 known splicing junctions. 481689 novel splicing junctions.
sampling 95% (358333907) splicing reads. 720909 splicing junctions. 234125 known splicing junctions. 486784 novel splicing junctions.
sampling 100% (377193587) splicing reads. 725247 splicing junctions. 234343 known splicing junctions. 490904 novel splicing junctions.