[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (20156095) splicing reads. 281947 splicing junctions. 186034 known splicing junctions. 95913 novel splicing junctions.
sampling 10% (40312191) splicing reads. 356818 splicing junctions. 199609 known splicing junctions. 157209 novel splicing junctions.
sampling 15% (60468287) splicing reads. 412289 splicing junctions. 207109 known splicing junctions. 205180 novel splicing junctions.
sampling 20% (80624382) splicing reads. 456983 splicing junctions. 211917 known splicing junctions. 245066 novel splicing junctions.
sampling 25% (100780478) splicing reads. 494798 splicing junctions. 215538 known splicing junctions. 279260 novel splicing junctions.
sampling 30% (120936574) splicing reads. 527448 splicing junctions. 218268 known splicing junctions. 309180 novel splicing junctions.
sampling 35% (141092669) splicing reads. 555609 splicing junctions. 220487 known splicing junctions. 335122 novel splicing junctions.
sampling 40% (161248765) splicing reads. 580214 splicing junctions. 222323 known splicing junctions. 357891 novel splicing junctions.
sampling 45% (181404861) splicing reads. 602128 splicing junctions. 223766 known splicing junctions. 378362 novel splicing junctions.
sampling 50% (201560957) splicing reads. 621173 splicing junctions. 224995 known splicing junctions. 396178 novel splicing junctions.
sampling 55% (221717052) splicing reads. 638435 splicing junctions. 226034 known splicing junctions. 412401 novel splicing junctions.
sampling 60% (241873148) splicing reads. 653868 splicing junctions. 226996 known splicing junctions. 426872 novel splicing junctions.
sampling 65% (262029244) splicing reads. 667637 splicing junctions. 227831 known splicing junctions. 439806 novel splicing junctions.
sampling 70% (282185339) splicing reads. 679914 splicing junctions. 228544 known splicing junctions. 451370 novel splicing junctions.
sampling 75% (302341435) splicing reads. 690884 splicing junctions. 229183 known splicing junctions. 461701 novel splicing junctions.
sampling 80% (322497531) splicing reads. 700585 splicing junctions. 229712 known splicing junctions. 470873 novel splicing junctions.
sampling 85% (342653626) splicing reads. 709261 splicing junctions. 230212 known splicing junctions. 479049 novel splicing junctions.
sampling 90% (362809722) splicing reads. 716711 splicing junctions. 230612 known splicing junctions. 486099 novel splicing junctions.
sampling 95% (382965818) splicing reads. 722983 splicing junctions. 230968 known splicing junctions. 492015 novel splicing junctions.
sampling 100% (403121914) splicing reads. 728404 splicing junctions. 231241 known splicing junctions. 497163 novel splicing junctions.