[W::hts_idx_load3] The index file is older than the data file: 659_bQZ-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	331388210
Known Splicing Events:	327863658
Partial Novel Splicing Events:	1784631
Novel Splicing Events:	1597496
Filtered Splicing Events:	142425

Total splicing  Junctions:	599053
Known Splicing Junctions:	285403
Partial Novel Splicing Junctions:	166669
Novel Splicing Junctions:	146981

===================================================================
Create BED file ...
Create Interact file ...