[W::hts_idx_load3] The index file is older than the data file: 659_bhK-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (19758277) splicing reads. 261809 splicing junctions. 185567 known splicing junctions. 76242 novel splicing junctions.
sampling 10% (39516554) splicing reads. 320195 splicing junctions. 198309 known splicing junctions. 121886 novel splicing junctions.
sampling 15% (59274831) splicing reads. 361494 splicing junctions. 205256 known splicing junctions. 156238 novel splicing junctions.
sampling 20% (79033108) splicing reads. 393359 splicing junctions. 209748 known splicing junctions. 183611 novel splicing junctions.
sampling 25% (98791385) splicing reads. 419429 splicing junctions. 213031 known splicing junctions. 206398 novel splicing junctions.
sampling 30% (118549662) splicing reads. 441516 splicing junctions. 215672 known splicing junctions. 225844 novel splicing junctions.
sampling 35% (138307939) splicing reads. 460340 splicing junctions. 217657 known splicing junctions. 242683 novel splicing junctions.
sampling 40% (158066216) splicing reads. 476479 splicing junctions. 219323 known splicing junctions. 257156 novel splicing junctions.
sampling 45% (177824493) splicing reads. 490624 splicing junctions. 220718 known splicing junctions. 269906 novel splicing junctions.
sampling 50% (197582770) splicing reads. 502871 splicing junctions. 221865 known splicing junctions. 281006 novel splicing junctions.
sampling 55% (217341047) splicing reads. 514051 splicing junctions. 222853 known splicing junctions. 291198 novel splicing junctions.
sampling 60% (237099324) splicing reads. 523663 splicing junctions. 223689 known splicing junctions. 299974 novel splicing junctions.
sampling 65% (256857601) splicing reads. 532245 splicing junctions. 224414 known splicing junctions. 307831 novel splicing junctions.
sampling 70% (276615878) splicing reads. 539805 splicing junctions. 225009 known splicing junctions. 314796 novel splicing junctions.
sampling 75% (296374155) splicing reads. 546490 splicing junctions. 225517 known splicing junctions. 320973 novel splicing junctions.
sampling 80% (316132432) splicing reads. 552328 splicing junctions. 226014 known splicing junctions. 326314 novel splicing junctions.
sampling 85% (335890709) splicing reads. 557520 splicing junctions. 226421 known splicing junctions. 331099 novel splicing junctions.
sampling 90% (355648986) splicing reads. 561914 splicing junctions. 226767 known splicing junctions. 335147 novel splicing junctions.
sampling 95% (375407263) splicing reads. 565675 splicing junctions. 227080 known splicing junctions. 338595 novel splicing junctions.
sampling 100% (395165540) splicing reads. 568997 splicing junctions. 227341 known splicing junctions. 341656 novel splicing junctions.