[W::hts_idx_load3] The index file is older than the data file: 659_6i-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9438073) splicing reads. 238950 splicing junctions. 180812 known splicing junctions. 58138 novel splicing junctions.
sampling 10% (18876146) splicing reads. 284393 splicing junctions. 192630 known splicing junctions. 91763 novel splicing junctions.
sampling 15% (28314219) splicing reads. 314698 splicing junctions. 198710 known splicing junctions. 115988 novel splicing junctions.
sampling 20% (37752292) splicing reads. 336862 splicing junctions. 202642 known splicing junctions. 134220 novel splicing junctions.
sampling 25% (47190365) splicing reads. 353953 splicing junctions. 205201 known splicing junctions. 148752 novel splicing junctions.
sampling 30% (56628438) splicing reads. 367824 splicing junctions. 207143 known splicing junctions. 160681 novel splicing junctions.
sampling 35% (66066510) splicing reads. 379181 splicing junctions. 208588 known splicing junctions. 170593 novel splicing junctions.
sampling 40% (75504584) splicing reads. 388651 splicing junctions. 209771 known splicing junctions. 178880 novel splicing junctions.
sampling 45% (84942657) splicing reads. 396550 splicing junctions. 210737 known splicing junctions. 185813 novel splicing junctions.
sampling 50% (94380730) splicing reads. 403104 splicing junctions. 211566 known splicing junctions. 191538 novel splicing junctions.
sampling 55% (103818803) splicing reads. 408760 splicing junctions. 212219 known splicing junctions. 196541 novel splicing junctions.
sampling 60% (113256876) splicing reads. 413498 splicing junctions. 212727 known splicing junctions. 200771 novel splicing junctions.
sampling 65% (122694949) splicing reads. 417600 splicing junctions. 213234 known splicing junctions. 204366 novel splicing junctions.
sampling 70% (132133021) splicing reads. 421053 splicing junctions. 213590 known splicing junctions. 207463 novel splicing junctions.
sampling 75% (141571095) splicing reads. 424037 splicing junctions. 213917 known splicing junctions. 210120 novel splicing junctions.
sampling 80% (151009168) splicing reads. 426556 splicing junctions. 214200 known splicing junctions. 212356 novel splicing junctions.
sampling 85% (160447241) splicing reads. 428505 splicing junctions. 214410 known splicing junctions. 214095 novel splicing junctions.
sampling 90% (169885314) splicing reads. 430044 splicing junctions. 214580 known splicing junctions. 215464 novel splicing junctions.
sampling 95% (179323387) splicing reads. 431198 splicing junctions. 214702 known splicing junctions. 216496 novel splicing junctions.
sampling 100% (188761460) splicing reads. 431969 splicing junctions. 214796 known splicing junctions. 217173 novel splicing junctions.