+ mkdir 659_bCu-T1-TRNA-1_A23MVY2LT4_1 + all_qc -bam 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_bCu-T1-TRNA-1_A23MVY2LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai 0it [00:00, ?it/s] 155517it [00:00, 1555101.04it/s] 321794it [00:00, 1618394.62it/s] 492820it [00:00, 1660219.12it/s] 660428it [00:00, 1666336.79it/s] 835299it [00:00, 1696014.21it/s] 1007644it [00:00, 1705176.44it/s] 1180646it [00:00, 1713273.97it/s] 1355674it [00:00, 1725032.18it/s] 1531759it [00:00, 1736068.69it/s] 1707083it [00:01, 1741313.37it/s] 1881964it [00:01, 1743583.83it/s] 2056323it [00:01, 1715684.48it/s] 2230037it [00:01, 1722088.03it/s] 2408532it [00:01, 1740903.56it/s] 2587084it [00:01, 1754258.30it/s] 2762565it [00:01, 1747315.74it/s] 2937339it [00:01, 1744614.68it/s] 3111830it [00:01, 1631645.26it/s] 3279326it [00:01, 1643988.79it/s] 3444812it [00:02, 1634258.81it/s] 3608982it [00:02, 1635742.86it/s] 3778818it [00:02, 1654084.55it/s] 3951307it [00:02, 1674966.65it/s] 4127185it [00:02, 1699815.59it/s] 4297417it [00:02, 1665304.04it/s] 4464258it [00:02, 1605198.50it/s] 4625369it [00:02, 1561668.07it/s] 4785350it [00:02, 1572529.68it/s] 4950743it [00:02, 1596062.11it/s] 5113861it [00:03, 1606313.02it/s] 5274783it [00:03, 1603384.87it/s] 5442159it [00:03, 1624188.10it/s] 5609484it [00:03, 1638620.73it/s] 5773478it [00:03, 1632078.30it/s] 5944592it [00:03, 1655547.92it/s] 6121177it [00:03, 1688427.14it/s] 6296930it [00:03, 1709053.81it/s] 6470456it [00:03, 1716866.89it/s] 6642188it [00:03, 1714759.15it/s] 6816166it [00:04, 1722224.50it/s] 6991049it [00:04, 1730169.34it/s] 7167818it [00:04, 1741346.30it/s] 7345252it [00:04, 1751072.98it/s] 7520370it [00:04, 1740385.71it/s] 7694431it [00:04, 1736761.43it/s] 7869445it [00:04, 1740728.97it/s] 8043531it [00:04, 1738415.38it/s] 8219128it [00:04, 1743510.70it/s] 8395090it [00:04, 1748233.93it/s][W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai 8569920it [00:05, 1745320.62it/s] 8744458it [00:05, 1735092.42it/s] 8917983it [00:05, 1658601.36it/s] 9084533it [00:05, 1492748.53it/s] 9237103it [00:05, 1466959.08it/s] 9401550it [00:05, 1515641.54it/s] 9556209it [00:05, 1524326.12it/s] 9718208it [00:05, 1551727.13it/s] 9881555it [00:05, 1575456.98it/s] 10054466it [00:06, 1620513.72it/s] 10217231it [00:06, 1598703.74it/s][W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai 10389973it [00:06, 1636466.44it/s] 10554080it [00:06, 1636892.01it/s] 10728193it [00:06, 1667788.67it/s] 10895232it [00:06, 1623542.81it/s] 11062158it [00:06, 1636894.56it/s] 11227365it [00:06, 1641335.92it/s] 11391733it [00:06, 1640503.31it/s] 11469948it [00:06, 1662986.40it/s] [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.md.bai + merge_qc -folders 659_bCu-T1-TRNA-1_A23MVY2LT4_1/659_bCu-T1-TRNA-1_A23MVY2LT4_1.md -output 659_bCu-T1-TRNA-1_A23MVY2LT4_1/659_bCu-T1-TRNA-1_A23MVY2LT4_1_full_basic_stats.tsv + head -n1 659_bCu-T1-TRNA-1_A23MVY2LT4_1/659_bCu-T1-TRNA-1_A23MVY2LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_bCu-T1-TRNA-1_A23MVY2LT4_1/659_bCu-T1-TRNA-1_A23MVY2LT4_1_full_basic_stats.tsv