[W::hts_idx_load3] The index file is older than the data file: 659_bGc-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17160092) splicing reads. 276175 splicing junctions. 191970 known splicing junctions. 84205 novel splicing junctions.
sampling 10% (34320184) splicing reads. 329127 splicing junctions. 202706 known splicing junctions. 126421 novel splicing junctions.
sampling 15% (51480277) splicing reads. 362185 splicing junctions. 207874 known splicing junctions. 154311 novel splicing junctions.
sampling 20% (68640369) splicing reads. 385879 splicing junctions. 211217 known splicing junctions. 174662 novel splicing junctions.
sampling 25% (85800462) splicing reads. 403449 splicing junctions. 213543 known splicing junctions. 189906 novel splicing junctions.
sampling 30% (102960554) splicing reads. 416992 splicing junctions. 215142 known splicing junctions. 201850 novel splicing junctions.
sampling 35% (120120647) splicing reads. 427866 splicing junctions. 216324 known splicing junctions. 211542 novel splicing junctions.
sampling 40% (137280739) splicing reads. 436754 splicing junctions. 217199 known splicing junctions. 219555 novel splicing junctions.
sampling 45% (154440832) splicing reads. 444027 splicing junctions. 217972 known splicing junctions. 226055 novel splicing junctions.
sampling 50% (171600924) splicing reads. 449940 splicing junctions. 218537 known splicing junctions. 231403 novel splicing junctions.
sampling 55% (188761016) splicing reads. 455079 splicing junctions. 219029 known splicing junctions. 236050 novel splicing junctions.
sampling 60% (205921109) splicing reads. 459424 splicing junctions. 219455 known splicing junctions. 239969 novel splicing junctions.
sampling 65% (223081201) splicing reads. 463198 splicing junctions. 219816 known splicing junctions. 243382 novel splicing junctions.
sampling 70% (240241294) splicing reads. 466402 splicing junctions. 220149 known splicing junctions. 246253 novel splicing junctions.
sampling 75% (257401386) splicing reads. 469066 splicing junctions. 220386 known splicing junctions. 248680 novel splicing junctions.
sampling 80% (274561479) splicing reads. 471299 splicing junctions. 220607 known splicing junctions. 250692 novel splicing junctions.
sampling 85% (291721571) splicing reads. 473148 splicing junctions. 220777 known splicing junctions. 252371 novel splicing junctions.
sampling 90% (308881664) splicing reads. 474670 splicing junctions. 220925 known splicing junctions. 253745 novel splicing junctions.
sampling 95% (326041756) splicing reads. 475834 splicing junctions. 221016 known splicing junctions. 254818 novel splicing junctions.
sampling 100% (343201849) splicing reads. 476697 splicing junctions. 221096 known splicing junctions. 255601 novel splicing junctions.