[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	403261926
Known Splicing Events:	400189941
Partial Novel Splicing Events:	1690318
Novel Splicing Events:	1243866
Filtered Splicing Events:	137801

Total splicing  Junctions:	728540
Known Splicing Junctions:	299511
Partial Novel Splicing Junctions:	202331
Novel Splicing Junctions:	226698

===================================================================
Create BED file ...
Create Interact file ...