[W::hts_idx_load3] The index file is older than the data file: 659_buD-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14071594) splicing reads. 268015 splicing junctions. 188036 known splicing junctions. 79979 novel splicing junctions.
sampling 10% (28143189) splicing reads. 329260 splicing junctions. 200437 known splicing junctions. 128823 novel splicing junctions.
sampling 15% (42214784) splicing reads. 372281 splicing junctions. 207167 known splicing junctions. 165114 novel splicing junctions.
sampling 20% (56286378) splicing reads. 405010 splicing junctions. 211431 known splicing junctions. 193579 novel splicing junctions.
sampling 25% (70357973) splicing reads. 431421 splicing junctions. 214529 known splicing junctions. 216892 novel splicing junctions.
sampling 30% (84429568) splicing reads. 453133 splicing junctions. 216882 known splicing junctions. 236251 novel splicing junctions.
sampling 35% (98501162) splicing reads. 471397 splicing junctions. 218663 known splicing junctions. 252734 novel splicing junctions.
sampling 40% (112572757) splicing reads. 487080 splicing junctions. 220130 known splicing junctions. 266950 novel splicing junctions.
sampling 45% (126644352) splicing reads. 500361 splicing junctions. 221339 known splicing junctions. 279022 novel splicing junctions.
sampling 50% (140715947) splicing reads. 511822 splicing junctions. 222331 known splicing junctions. 289491 novel splicing junctions.
sampling 55% (154787541) splicing reads. 521947 splicing junctions. 223132 known splicing junctions. 298815 novel splicing junctions.
sampling 60% (168859136) splicing reads. 530795 splicing junctions. 223859 known splicing junctions. 306936 novel splicing junctions.
sampling 65% (182930731) splicing reads. 538407 splicing junctions. 224420 known splicing junctions. 313987 novel splicing junctions.
sampling 70% (197002325) splicing reads. 545173 splicing junctions. 224935 known splicing junctions. 320238 novel splicing junctions.
sampling 75% (211073920) splicing reads. 550955 splicing junctions. 225344 known splicing junctions. 325611 novel splicing junctions.
sampling 80% (225145515) splicing reads. 555857 splicing junctions. 225707 known splicing junctions. 330150 novel splicing junctions.
sampling 85% (239217109) splicing reads. 560060 splicing junctions. 226013 known splicing junctions. 334047 novel splicing junctions.
sampling 90% (253288704) splicing reads. 563732 splicing junctions. 226294 known splicing junctions. 337438 novel splicing junctions.
sampling 95% (267360299) splicing reads. 566654 splicing junctions. 226519 known splicing junctions. 340135 novel splicing junctions.
sampling 100% (281431894) splicing reads. 568985 splicing junctions. 226696 known splicing junctions. 342289 novel splicing junctions.