[W::hts_idx_load3] The index file is older than the data file: 659_6i-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10231035) splicing reads. 234474 splicing junctions. 179415 known splicing junctions. 55059 novel splicing junctions.
sampling 10% (20462070) splicing reads. 270237 splicing junctions. 189041 known splicing junctions. 81196 novel splicing junctions.
sampling 15% (30693106) splicing reads. 291144 splicing junctions. 193662 known splicing junctions. 97482 novel splicing junctions.
sampling 20% (40924141) splicing reads. 305265 splicing junctions. 196438 known splicing junctions. 108827 novel splicing junctions.
sampling 25% (51155177) splicing reads. 315163 splicing junctions. 198213 known splicing junctions. 116950 novel splicing junctions.
sampling 30% (61386212) splicing reads. 322691 splicing junctions. 199443 known splicing junctions. 123248 novel splicing junctions.
sampling 35% (71617248) splicing reads. 328549 splicing junctions. 200349 known splicing junctions. 128200 novel splicing junctions.
sampling 40% (81848283) splicing reads. 333300 splicing junctions. 201105 known splicing junctions. 132195 novel splicing junctions.
sampling 45% (92079319) splicing reads. 337102 splicing junctions. 201681 known splicing junctions. 135421 novel splicing junctions.
sampling 50% (102310354) splicing reads. 340315 splicing junctions. 202121 known splicing junctions. 138194 novel splicing junctions.
sampling 55% (112541389) splicing reads. 342947 splicing junctions. 202508 known splicing junctions. 140439 novel splicing junctions.
sampling 60% (122772425) splicing reads. 345196 splicing junctions. 202832 known splicing junctions. 142364 novel splicing junctions.
sampling 65% (133003460) splicing reads. 347055 splicing junctions. 203068 known splicing junctions. 143987 novel splicing junctions.
sampling 70% (143234496) splicing reads. 348571 splicing junctions. 203295 known splicing junctions. 145276 novel splicing junctions.
sampling 75% (153465531) splicing reads. 349897 splicing junctions. 203464 known splicing junctions. 146433 novel splicing junctions.
sampling 80% (163696567) splicing reads. 350918 splicing junctions. 203602 known splicing junctions. 147316 novel splicing junctions.
sampling 85% (173927602) splicing reads. 351778 splicing junctions. 203738 known splicing junctions. 148040 novel splicing junctions.
sampling 90% (184158638) splicing reads. 352442 splicing junctions. 203832 known splicing junctions. 148610 novel splicing junctions.
sampling 95% (194389673) splicing reads. 352966 splicing junctions. 203899 known splicing junctions. 149067 novel splicing junctions.
sampling 100% (204620709) splicing reads. 353310 splicing junctions. 203947 known splicing junctions. 149363 novel splicing junctions.