Done. -parsing GTF file: /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.v4W9t1d1P2/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1_1.fastp.fastq.gz /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam 659_bQZ-T1-TRNA-1_A23MVY2LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index 659_bQZ-T1-TRNA-1_A23MVY2LT4_1.star.sortedByCoord.out.bam 11:57:01 : INFO : Done. -parsing GTF file: /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.gtf -parsing /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] -done parsing /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 270, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 44, ' ** passed ** ' => 1601, 'excessive soft clipping' => 2839, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 17, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 27, 'per_id < 96' => 1691, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 14 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] -fusion SPANNING read extraction for scaff: AGAP3--BRAF -fusion SPANNING read extraction for scaff: GUCY1B2--NKAIN2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] -fusion SPANNING read extraction for scaff: SMARCD3--MGAM [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] [194000] [195000] [196000] [197000] [198000] [199000] [200000] [201000] -fusion SPANNING read extraction for scaff: SYNE2--MTHFD1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 3539 }; EM: Starting log likelihood: -1412.711445 EM: Round [1] log likelihood: -1410.566673 EM: Round [2] log likelihood: -1410.565392 EM: Round [3] log likelihood: -1410.565392 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam into /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.read_coords > /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.frag_coords > /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.v4W9t1d1P2/fi_workdir/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -1381.078766 EM: Round [1] log likelihood: -1380.704178 EM: Round [2] log likelihood: -1380.704161 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1_1.fastp.fastq.gz: 270100702 Warning - not locating file: /tmp/nxf.v4W9t1d1P2/659_bQZ-T1-TRNA-1_A23MVY2LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000123201.14] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] ls: cannot access 'IGV_inputs': No such file or directory