Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1a/7177bb07b93c31cc7733a6eaf14c86/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/26/184fb834bc8193db29cca1fd0a720b/659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/26/184fb834bc8193db29cca1fd0a720b/659_bua-T1-TRNA-1_A23MVY2LT4_1.SJ.out.tab
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/26/184fb834bc8193db29cca1fd0a720b/659_bua-T1-TRNA-1_A23MVY2LT4_1.Chimeric.out.junction
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c7/cece951fc75f7526dda2fbbc39d89c/659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1a/7177bb07b93c31cc7733a6eaf14c86/.command.run
==> STAGING COMPLETE (7 inputs)
08:59:36 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[9830000], rate=10347368.42/min 09:00:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-659_bua-T1-TRNA-1_A23MVY2LT4_1.introns: identified 7036 cancer introns
09:00:47 : INFO : Execution Time = 0.02 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns.prelim
09:00:47 : INFO : Running: touch /tmp/nxf.UT5N8JDCwj/659_bua-T1-TRNA-1_A23MVY2LT4_1.chckpts/prelim_introns.ok
09:00:47 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns
09:00:47 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns.prelim --min_total_reads 5 > 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns
09:00:47 : INFO : Running: touch /tmp/nxf.UT5N8JDCwj/659_bua-T1-TRNA-1_A23MVY2LT4_1.chckpts/introns_filtered.ok
09:00:47 : INFO : -found 3144 cancer introns
09:00:47 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns --cancer_introns 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns.for_IGV.bed
09:00:48 : INFO : Creating the BED File.
09:00:57 : INFO : Saving Bed File as 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns.for_IGV.bed
09:00:57 : INFO : Execution Time = 0.17 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns --cancer_introns 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns.for_IGV.bed
09:00:57 : INFO : Running: touch /tmp/nxf.UT5N8JDCwj/659_bua-T1-TRNA-1_A23MVY2LT4_1.chckpts/intron_igv_bed.ok
09:00:58 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns.for_IGV.bed --bam 659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_bua-T1-TRNA-1_A23MVY2LT4_1
[W::hts_idx_load3] The index file is older than the data file: 659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
09:32:29 : INFO : Execution Time = 31.52 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 659_bua-T1-TRNA-1_A23MVY2LT4_1.introns.for_IGV.bed --bam 659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam --output_prefix 659_bua-T1-TRNA-1_A23MVY2LT4_1
09:32:29 : INFO : Running: touch /tmp/nxf.UT5N8JDCwj/659_bua-T1-TRNA-1_A23MVY2LT4_1.chckpts/reads_alignments_extracted.ok
09:32:29 : INFO : Running: samtools sort -o 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer_intron_reads.sorted.bam 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer_intron_reads.bam
09:32:30 : INFO : Execution Time = 0.02 minutes. CMD: samtools sort -o 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer_intron_reads.sorted.bam 659_bua-T1-TRNA-1_A23MVY2LT4_1.cancer_intron_reads.bam
09:32:30 : INFO : Running: touch /tmp/nxf.UT5N8JDCwj/659_bua-T1-TRNA-1_A23MVY2LT4_1.chckpts/sort_cancer_intron_reads.ok
09:32:30 : INFO : Running: samtools sort -o 659_bua-T1-TRNA-1_A23MVY2LT4_1.gene_reads.sorted.bam 659_bua-T1-TRNA-1_A23MVY2LT4_1.gene_reads.bam