Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c7/5e87ad9f2b92038a203a50ec5061e2/659_bNS-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1a/f73a0e22861201cc7c5cccf07ed1b4/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/4b9acc3ad4acc9690d7fed1a19a938/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/81/486aec410fed2a0a5941a018ee2bde/659_bNS-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/4b9acc3ad4acc9690d7fed1a19a938/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 659_bNS-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18644832) splicing reads. 284066 splicing junctions. 194072 known splicing junctions. 89994 novel splicing junctions.
sampling 10% (37289664) splicing reads. 347635 splicing junctions. 206368 known splicing junctions. 141267 novel splicing junctions.
sampling 15% (55934497) splicing reads. 391242 splicing junctions. 212685 known splicing junctions. 178557 novel splicing junctions.
sampling 20% (74579329) splicing reads. 424439 splicing junctions. 216649 known splicing junctions. 207790 novel splicing junctions.
sampling 25% (93224162) splicing reads. 450471 splicing junctions. 219522 known splicing junctions. 230949 novel splicing junctions.
sampling 30% (111868994) splicing reads. 472170 splicing junctions. 221697 known splicing junctions. 250473 novel splicing junctions.
sampling 35% (130513826) splicing reads. 490157 splicing junctions. 223327 known splicing junctions. 266830 novel splicing junctions.
sampling 40% (149158659) splicing reads. 505166 splicing junctions. 224639 known splicing junctions. 280527 novel splicing junctions.
sampling 45% (167803491) splicing reads. 518286 splicing junctions. 225695 known splicing junctions. 292591 novel splicing junctions.
sampling 50% (186448324) splicing reads. 529410 splicing junctions. 226577 known splicing junctions. 302833 novel splicing junctions.
sampling 55% (205093156) splicing reads. 539033 splicing junctions. 227333 known splicing junctions. 311700 novel splicing junctions.
sampling 60% (223737988) splicing reads. 547382 splicing junctions. 227951 known splicing junctions. 319431 novel splicing junctions.
sampling 65% (242382821) splicing reads. 554682 splicing junctions. 228487 known splicing junctions. 326195 novel splicing junctions.
sampling 70% (261027653) splicing reads. 561067 splicing junctions. 228961 known splicing junctions. 332106 novel splicing junctions.
sampling 75% (279672486) splicing reads. 566546 splicing junctions. 229341 known splicing junctions. 337205 novel splicing junctions.
sampling 80% (298317318) splicing reads. 571362 splicing junctions. 229670 known splicing junctions. 341692 novel splicing junctions.
sampling 85% (316962150) splicing reads. 575505 splicing junctions. 229950 known splicing junctions. 345555 novel splicing junctions.
sampling 90% (335606983) splicing reads. 579035 splicing junctions. 230195 known splicing junctions. 348840 novel splicing junctions.
sampling 95% (354251815) splicing reads. 582016 splicing junctions. 230398 known splicing junctions. 351618 novel splicing junctions.
sampling 100% (372896648) splicing reads. 584462 splicing junctions. 230574 known splicing junctions. 353888 novel splicing junctions.
null device 
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