[W::hts_idx_load3] The index file is older than the data file: 659_bNS-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	373061638
Known Splicing Events:	369638475
Partial Novel Splicing Events:	1868833
Novel Splicing Events:	1395579
Filtered Splicing Events:	158751

Total splicing  Junctions:	584637
Known Splicing Junctions:	277090
Partial Novel Splicing Junctions:	156431
Novel Splicing Junctions:	151116

===================================================================
Create BED file ...
Create Interact file ...