[W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	220603188
Known Splicing Events:	218218538
Partial Novel Splicing Events:	1436385
Novel Splicing Events:	833743
Filtered Splicing Events:	114522

Total splicing  Junctions:	312980
Known Splicing Junctions:	208646
Partial Novel Splicing Junctions:	58901
Novel Splicing Junctions:	45433

===================================================================
Create BED file ...
Create Interact file ...