[W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11024258) splicing reads. 217429 splicing junctions. 164666 known splicing junctions. 52763 novel splicing junctions.
sampling 10% (22048516) splicing reads. 248316 splicing junctions. 174032 known splicing junctions. 74284 novel splicing junctions.
sampling 15% (33072775) splicing reads. 265220 splicing junctions. 178183 known splicing junctions. 87037 novel splicing junctions.
sampling 20% (44097033) splicing reads. 275930 splicing junctions. 180538 known splicing junctions. 95392 novel splicing junctions.
sampling 25% (55121292) splicing reads. 283595 splicing junctions. 182063 known splicing junctions. 101532 novel splicing junctions.
sampling 30% (66145550) splicing reads. 289246 splicing junctions. 183163 known splicing junctions. 106083 novel splicing junctions.
sampling 35% (77169808) splicing reads. 293697 splicing junctions. 183911 known splicing junctions. 109786 novel splicing junctions.
sampling 40% (88194067) splicing reads. 297264 splicing junctions. 184531 known splicing junctions. 112733 novel splicing junctions.
sampling 45% (99218325) splicing reads. 300184 splicing junctions. 185035 known splicing junctions. 115149 novel splicing junctions.
sampling 50% (110242584) splicing reads. 302597 splicing junctions. 185415 known splicing junctions. 117182 novel splicing junctions.
sampling 55% (121266842) splicing reads. 304720 splicing junctions. 185738 known splicing junctions. 118982 novel splicing junctions.
sampling 60% (132291100) splicing reads. 306380 splicing junctions. 186003 known splicing junctions. 120377 novel splicing junctions.
sampling 65% (143315359) splicing reads. 307784 splicing junctions. 186196 known splicing junctions. 121588 novel splicing junctions.
sampling 70% (154339617) splicing reads. 309055 splicing junctions. 186366 known splicing junctions. 122689 novel splicing junctions.
sampling 75% (165363876) splicing reads. 310040 splicing junctions. 186514 known splicing junctions. 123526 novel splicing junctions.
sampling 80% (176388134) splicing reads. 310901 splicing junctions. 186628 known splicing junctions. 124273 novel splicing junctions.
sampling 85% (187412392) splicing reads. 311630 splicing junctions. 186722 known splicing junctions. 124908 novel splicing junctions.
sampling 90% (198436651) splicing reads. 312200 splicing junctions. 186801 known splicing junctions. 125399 novel splicing junctions.
sampling 95% (209460909) splicing reads. 312568 splicing junctions. 186859 known splicing junctions. 125709 novel splicing junctions.
sampling 100% (220485168) splicing reads. 312824 splicing junctions. 186892 known splicing junctions. 125932 novel splicing junctions.