[W::hts_idx_load3] The index file is older than the data file: 659_bua-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18035085) splicing reads. 268518 splicing junctions. 179196 known splicing junctions. 89322 novel splicing junctions.
sampling 10% (36070171) splicing reads. 335427 splicing junctions. 192173 known splicing junctions. 143254 novel splicing junctions.
sampling 15% (54105256) splicing reads. 383641 splicing junctions. 199225 known splicing junctions. 184416 novel splicing junctions.
sampling 20% (72140342) splicing reads. 421491 splicing junctions. 203964 known splicing junctions. 217527 novel splicing junctions.
sampling 25% (90175428) splicing reads. 452624 splicing junctions. 207257 known splicing junctions. 245367 novel splicing junctions.
sampling 30% (108210513) splicing reads. 479044 splicing junctions. 209770 known splicing junctions. 269274 novel splicing junctions.
sampling 35% (126245599) splicing reads. 501473 splicing junctions. 211811 known splicing junctions. 289662 novel splicing junctions.
sampling 40% (144280685) splicing reads. 520938 splicing junctions. 213395 known splicing junctions. 307543 novel splicing junctions.
sampling 45% (162315770) splicing reads. 537782 splicing junctions. 214757 known splicing junctions. 323025 novel splicing junctions.
sampling 50% (180350856) splicing reads. 552723 splicing junctions. 215954 known splicing junctions. 336769 novel splicing junctions.
sampling 55% (198385942) splicing reads. 565738 splicing junctions. 216921 known splicing junctions. 348817 novel splicing junctions.
sampling 60% (216421027) splicing reads. 577218 splicing junctions. 217796 known splicing junctions. 359422 novel splicing junctions.
sampling 65% (234456113) splicing reads. 587253 splicing junctions. 218486 known splicing junctions. 368767 novel splicing junctions.
sampling 70% (252491199) splicing reads. 596077 splicing junctions. 219090 known splicing junctions. 376987 novel splicing junctions.
sampling 75% (270526284) splicing reads. 603878 splicing junctions. 219662 known splicing junctions. 384216 novel splicing junctions.
sampling 80% (288561370) splicing reads. 610612 splicing junctions. 220120 known splicing junctions. 390492 novel splicing junctions.
sampling 85% (306596456) splicing reads. 616427 splicing junctions. 220506 known splicing junctions. 395921 novel splicing junctions.
sampling 90% (324631541) splicing reads. 621259 splicing junctions. 220829 known splicing junctions. 400430 novel splicing junctions.
sampling 95% (342666627) splicing reads. 625450 splicing junctions. 221103 known splicing junctions. 404347 novel splicing junctions.
sampling 100% (360701713) splicing reads. 628676 splicing junctions. 221321 known splicing junctions. 407355 novel splicing junctions.