[W::hts_idx_load3] The index file is older than the data file: 659_buD-T1-TRNA-1_A23MVY2LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18355205) splicing reads. 295042 splicing junctions. 193955 known splicing junctions. 101087 novel splicing junctions.
sampling 10% (36710411) splicing reads. 369906 splicing junctions. 206851 known splicing junctions. 163055 novel splicing junctions.
sampling 15% (55065617) splicing reads. 424540 splicing junctions. 213684 known splicing junctions. 210856 novel splicing junctions.
sampling 20% (73420823) splicing reads. 467878 splicing junctions. 218326 known splicing junctions. 249552 novel splicing junctions.
sampling 25% (91776029) splicing reads. 503137 splicing junctions. 221605 known splicing junctions. 281532 novel splicing junctions.
sampling 30% (110131234) splicing reads. 533101 splicing junctions. 224123 known splicing junctions. 308978 novel splicing junctions.
sampling 35% (128486440) splicing reads. 558922 splicing junctions. 226094 known splicing junctions. 332828 novel splicing junctions.
sampling 40% (146841646) splicing reads. 581050 splicing junctions. 227655 known splicing junctions. 353395 novel splicing junctions.
sampling 45% (165196852) splicing reads. 600659 splicing junctions. 228965 known splicing junctions. 371694 novel splicing junctions.
sampling 50% (183552058) splicing reads. 617976 splicing junctions. 230066 known splicing junctions. 387910 novel splicing junctions.
sampling 55% (201907263) splicing reads. 632849 splicing junctions. 231013 known splicing junctions. 401836 novel splicing junctions.
sampling 60% (220262469) splicing reads. 646099 splicing junctions. 231825 known splicing junctions. 414274 novel splicing junctions.
sampling 65% (238617675) splicing reads. 657790 splicing junctions. 232503 known splicing junctions. 425287 novel splicing junctions.
sampling 70% (256972881) splicing reads. 668036 splicing junctions. 233100 known splicing junctions. 434936 novel splicing junctions.
sampling 75% (275328087) splicing reads. 676842 splicing junctions. 233588 known splicing junctions. 443254 novel splicing junctions.
sampling 80% (293683292) splicing reads. 684596 splicing junctions. 234048 known splicing junctions. 450548 novel splicing junctions.
sampling 85% (312038498) splicing reads. 691236 splicing junctions. 234422 known splicing junctions. 456814 novel splicing junctions.
sampling 90% (330393704) splicing reads. 696759 splicing junctions. 234710 known splicing junctions. 462049 novel splicing junctions.
sampling 95% (348748910) splicing reads. 701389 splicing junctions. 234974 known splicing junctions. 466415 novel splicing junctions.
sampling 100% (367104116) splicing reads. 705018 splicing junctions. 235213 known splicing junctions. 469805 novel splicing junctions.