[W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_A23MVY2LT4_2.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	102598434
Known Splicing Events:	101487517
Partial Novel Splicing Events:	669091
Novel Splicing Events:	386606
Filtered Splicing Events:	55220

Total splicing  Junctions:	231210
Known Splicing Junctions:	178586
Partial Novel Splicing Junctions:	31012
Novel Splicing Junctions:	21612

===================================================================
Create BED file ...
Create Interact file ...