FastQCFastQC Report
Sat 16 May 2026
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165299
Total Bases24.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGT6029536.476324720657715TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGG3499521.170726985644198TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTGGG2591715.678860731159899TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTGGT2151713.01701764680972TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGTG6860.4150055354236868TruSeq Adapter, Index 4 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGGG5440.3291005995196583TruSeq Adapter, Index 4 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTGGGG5100.30853181204967967TruSeq Adapter, Index 4 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGTGTG4030.24380062795298216TruSeq Adapter, Index 4 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGTA3640.22020701879624194TruSeq Adapter, Index 4 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTGGTG3220.19479851662744482TruSeq Adapter, Index 4 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTGGGGGG2530.1530559773501352TruSeq Adapter, Index 4 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGGGGG2380.1439815122898505TruSeq Adapter, Index 4 (97% over 34bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGATCGGAAGAGCA2350.1421666192777936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCGAGG2330.14095669060308896TruSeq Adapter, Index 4 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCTAGTATG2180.13188222554280427TruSeq Adapter, Index 4 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATCGGGG2110.12764747518133807TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATGTGGG2080.12583258216928112TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCGGTGCTATGTAGG2040.12341272481987188TruSeq Adapter, Index 4 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1760.1064737233740071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)