Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 4457634 total bases: 673102734 Q20 bases: 637691797(94.7391%) Q30 bases: 587569925(87.2928%) Read2 before filtering: total reads: 4457634 total bases: 673102734 Q20 bases: 655214748(97.3425%) Q30 bases: 620187937(92.1387%) Read1 after filtering: total reads: 4362646 total bases: 404453033 Q20 bases: 402775533(99.5852%) Q30 bases: 394747520(97.6003%) Read2 after filtering: total reads: 4362646 total bases: 406418144 Q20 bases: 403841536(99.366%) Q30 bases: 394725698(97.1231%) Filtering result: reads passed filter: 8725292 reads failed due to low quality: 143074 reads failed due to too many N: 1390 reads failed due to too short: 45512 reads with adapter trimmed: 8451856 bases trimmed due to adapters: 512351889 Duplication rate: 10.7748% Insert size peak (evaluated by paired-end reads): 86 JSON report: 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1.fastp.json HTML report: 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1.fastp.html fastp --in1 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_1.fastq.gz --in2 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_2.fastq.gz --out1 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_1.fastp.fastq.gz --out2 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_2.fastp.fastq.gz --json 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1.fastp.json --html 1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 13 seconds