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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 6.80 256076560 3764221011
2 6.68 249916067 3743254453
3 6.99 259502348 3713846173
4 7.39 273892746 3706675031
5 7.41 272345315 3676440007
6 6.76 254194919 3761057280
7 6.56 248021330 3778753744
8 6.78 255971636 3774977755
2. SAMPLES WITH LOW READS (NON-NTC)
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Found 1 samples with low reads:
SampleID Index # Reads Lane(s)
20 1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2 TGTCGGTGCT-AGTTCTTCCG 165299 1,2,3,4,5,6,7,8
3. NTC SAMPLES WITH HIGH READS
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No NTC samples found with high reads
4. TOP UNKNOWN BARCODES
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Found 136 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 ACCAGGAATT GGGGGGGGGG 5754910 0.022473 0.001529 0.152884 0.019235
1 1 AGCCTCTGCT GGGGGGGGGG 5445921 0.021267 0.001447 0.144676 0.018202
2 1 GTGACGCACA GGGGGGGGGG 4425130 0.017280 0.001176 0.117558 0.014790
3 1 AGACGACAGA GGGGGGGGGG 4040373 0.015778 0.001073 0.107336 0.013504
4 1 ACTCGTCCAG GGGGGGGGGG 3980549 0.015544 0.001057 0.105747 0.013304
5 1 GAGTAGTGAC GGGGGGGGGG 3951009 0.015429 0.001050 0.104962 0.013206
6 1 TGGCACTGAT GGGGGGGGGG 3799569 0.014838 0.001009 0.100939 0.012699
7 1 TGAGTGTACA GGGGGGGGGG 3752965 0.014656 0.000997 0.099701 0.012544
8 1 GCATTCCTTC GGGGGGGGGG 3283868 0.012824 0.000872 0.087239 0.010976
9 1 CTCTATACTG GGGGGGGGGG 3041321 0.011877 0.000808 0.080795 0.010165
10 1 TCTCCGTACT GGGGGGGGGG 3040129 0.011872 0.000808 0.080764 0.010161
11 1 GCAGAAGTCT GGGGGGGGGG 2817544 0.011003 0.000749 0.074851 0.009417
12 1 GATAGGTGAA GGGGGGGGGG 2737065 0.010688 0.000727 0.072713 0.009148
13 1 TAGCGAGGCT GGGGGGGGGG 2362509 0.009226 0.000628 0.062762 0.007896
14 1 TCACCGTGGT GGGGGGGGGG 2201252 0.008596 0.000585 0.058478 0.007357
15 1 TTGCCGTATC GGGGGGGGGG 2026053 0.007912 0.000538 0.053824 0.006772
16 2 ACCAGGAATT GGGGGGGGGG 5518342 0.022081 0.001474 0.147421 0.018444
17 2 AGCCTCTGCT GGGGGGGGGG 5198858 0.020802 0.001389 0.138886 0.017376
18 2 GTGACGCACA GGGGGGGGGG 4256841 0.017033 0.001137 0.113720 0.014228
19 2 AGACGACAGA GGGGGGGGGG 3913329 0.015659 0.001045 0.104543 0.013080
20 2 ACTCGTCCAG GGGGGGGGGG 3823855 0.015301 0.001022 0.102153 0.012781
21 2 GAGTAGTGAC GGGGGGGGGG 3744352 0.014982 0.001000 0.100029 0.012515
22 2 TGGCACTGAT GGGGGGGGGG 3700076 0.014805 0.000988 0.098846 0.012367
23 2 TGAGTGTACA GGGGGGGGGG 3651655 0.014612 0.000976 0.097553 0.012205
24 2 GCATTCCTTC GGGGGGGGGG 3240950 0.012968 0.000866 0.086581 0.010832
25 2 TCTCCGTACT GGGGGGGGGG 2966452 0.011870 0.000792 0.079248 0.009915
26 2 CTCTATACTG GGGGGGGGGG 2943543 0.011778 0.000786 0.078636 0.009838
27 2 GCAGAAGTCT GGGGGGGGGG 2749993 0.011004 0.000735 0.073465 0.009191
28 2 GATAGGTGAA GGGGGGGGGG 2643038 0.010576 0.000706 0.070608 0.008834
29 2 TAGCGAGGCT GGGGGGGGGG 2293719 0.009178 0.000613 0.061276 0.007666
30 2 TCACCGTGGT GGGGGGGGGG 2152967 0.008615 0.000575 0.057516 0.007196
31 3 ACCAGGAATT GGGGGGGGGG 5754547 0.022175 0.001549 0.154948 0.019234
32 3 AGCCTCTGCT GGGGGGGGGG 4996013 0.019252 0.001345 0.134524 0.016698
33 3 GTGACGCACA GGGGGGGGGG 4234808 0.016319 0.001140 0.114028 0.014154
34 3 ACTCGTCCAG GGGGGGGGGG 4047358 0.015597 0.001090 0.108980 0.013528
35 3 TGGCACTGAT GGGGGGGGGG 3884212 0.014968 0.001046 0.104587 0.012982
36 3 GAGTAGTGAC GGGGGGGGGG 3867643 0.014904 0.001041 0.104141 0.012927
37 3 AGACGACAGA GGGGGGGGGG 3734697 0.014392 0.001006 0.100561 0.012483
38 3 TGAGTGTACA GGGGGGGGGG 3561319 0.013724 0.000959 0.095893 0.011903
39 3 TCTCCGTACT GGGGGGGGGG 3318618 0.012788 0.000894 0.089358 0.011092
40 3 GCATTCCTTC GGGGGGGGGG 3040510 0.011717 0.000819 0.081870 0.010162
41 3 CTCTATACTG GGGGGGGGGG 3035642 0.011698 0.000817 0.081738 0.010146
42 3 GCAGAAGTCT GGGGGGGGGG 2839537 0.010942 0.000765 0.076458 0.009491
43 3 TAGCGAGGCT GGGGGGGGGG 2537684 0.009779 0.000683 0.068330 0.008482
44 3 GATAGGTGAA GGGGGGGGGG 2518457 0.009705 0.000678 0.067813 0.008418
45 3 TTGCCGTATC GGGGGGGGGG 2328594 0.008973 0.000627 0.062700 0.007783
46 3 TCACCGTGGT GGGGGGGGGG 2088619 0.008049 0.000562 0.056239 0.006981
47 3 GCACTACCAG GGGGGGGGGG 1863200 0.007180 0.000502 0.050169 0.006227
48 4 ACCAGGAATT GGGGGGGGGG 5935969 0.021673 0.001601 0.160143 0.019840
49 4 AGCCTCTGCT GGGGGGGGGG 5005206 0.018274 0.001350 0.135032 0.016729
50 4 GTGACGCACA GGGGGGGGGG 4314983 0.015754 0.001164 0.116411 0.014422
51 4 TGGCACTGAT GGGGGGGGGG 4168021 0.015218 0.001124 0.112446 0.013931
52 4 ACTCGTCCAG GGGGGGGGGG 4116275 0.015029 0.001111 0.111050 0.013758
53 4 GAGTAGTGAC GGGGGGGGGG 3871275 0.014134 0.001044 0.104441 0.012939
54 4 AGACGACAGA GGGGGGGGGG 3754763 0.013709 0.001013 0.101297 0.012550
55 4 TGAGTGTACA GGGGGGGGGG 3578641 0.013066 0.000965 0.096546 0.011961
56 4 TCTCCGTACT GGGGGGGGGG 3528687 0.012883 0.000952 0.095198 0.011794
57 4 CTCTATACTG GGGGGGGGGG 3155066 0.011519 0.000851 0.085118 0.010545
58 4 GCAGAAGTCT GGGGGGGGGG 3056289 0.011159 0.000825 0.082454 0.010215
59 4 GCATTCCTTC GGGGGGGGGG 2992824 0.010927 0.000807 0.080741 0.010003
60 4 TAGCGAGGCT GGGGGGGGGG 2672706 0.009758 0.000721 0.072105 0.008933
61 4 GATAGGTGAA GGGGGGGGGG 2533806 0.009251 0.000684 0.068358 0.008469
62 4 TTGCCGTATC GGGGGGGGGG 2429398 0.008870 0.000655 0.065541 0.008120
63 4 GCACTACCAG GGGGGGGGGG 2169800 0.007922 0.000585 0.058538 0.007252
64 4 GATTGGCGAT GGGGGGGGGG 2137471 0.007804 0.000577 0.057665 0.007144
65 4 CCACTCAACG GGGGGGGGGG 2091394 0.007636 0.000564 0.056422 0.006990
66 4 TCACCGTGGT GGGGGGGGGG 2055667 0.007505 0.000555 0.055459 0.006871
67 5 ACCAGGAATT GGGGGGGGGG 5823993 0.021385 0.001584 0.158414 0.019466
68 5 AGCCTCTGCT GGGGGGGGGG 4820673 0.017701 0.001311 0.131123 0.016112
69 5 GTGACGCACA GGGGGGGGGG 4228057 0.015525 0.001150 0.115004 0.014132
70 5 ACTCGTCCAG GGGGGGGGGG 4045505 0.014854 0.001100 0.110039 0.013521
71 5 TGGCACTGAT GGGGGGGGGG 4005573 0.014708 0.001090 0.108952 0.013388
72 5 GAGTAGTGAC GGGGGGGGGG 3860216 0.014174 0.001050 0.104999 0.012902
73 5 AGACGACAGA GGGGGGGGGG 3615673 0.013276 0.000983 0.098347 0.012085
74 5 TGAGTGTACA GGGGGGGGGG 3582225 0.013153 0.000974 0.097437 0.011973
75 5 TCTCCGTACT GGGGGGGGGG 3414616 0.012538 0.000929 0.092878 0.011413
76 5 CTCTATACTG GGGGGGGGGG 3049855 0.011198 0.000830 0.082957 0.010194
77 5 GCATTCCTTC GGGGGGGGGG 2998867 0.011011 0.000816 0.081570 0.010023
78 5 GCAGAAGTCT GGGGGGGGGG 2879936 0.010575 0.000783 0.078335 0.009626
79 5 TAGCGAGGCT GGGGGGGGGG 2639442 0.009692 0.000718 0.071793 0.008822
80 5 TTGCCGTATC GGGGGGGGGG 2633074 0.009668 0.000716 0.071620 0.008801
81 5 GATAGGTGAA GGGGGGGGGG 2474170 0.009085 0.000673 0.067298 0.008269
82 5 TCACCGTGGT GGGGGGGGGG 2083146 0.007649 0.000567 0.056662 0.006963
83 5 GCACTACCAG GGGGGGGGGG 2012157 0.007388 0.000547 0.054731 0.006725
84 5 GATTGGCGAT GGGGGGGGGG 1971375 0.007239 0.000536 0.053622 0.006589
85 5 ATCCAGCATG AGATCTCGGT 1921932 0.007057 0.000523 0.052277 0.006424
86 5 CCACTCAACG GGGGGGGGGG 1920206 0.007051 0.000522 0.052230 0.006418
87 6 ACCAGGAATT GGGGGGGGGG 5700301 0.022425 0.001516 0.151561 0.019052
88 6 AGCCTCTGCT GGGGGGGGGG 5081395 0.019990 0.001351 0.135105 0.016984
89 6 GTGACGCACA GGGGGGGGGG 4214886 0.016581 0.001121 0.112067 0.014088
90 6 ACTCGTCCAG GGGGGGGGGG 3980604 0.015660 0.001058 0.105837 0.013305
91 6 GAGTAGTGAC GGGGGGGGGG 3840003 0.015107 0.001021 0.102099 0.012835
92 6 TGGCACTGAT GGGGGGGGGG 3814600 0.015007 0.001014 0.101424 0.012750
93 6 AGACGACAGA GGGGGGGGGG 3792318 0.014919 0.001008 0.100831 0.012675
94 6 TGAGTGTACA GGGGGGGGGG 3575856 0.014067 0.000951 0.095076 0.011952
95 6 TCTCCGTACT GGGGGGGGGG 3153155 0.012404 0.000838 0.083837 0.010539
96 6 GCATTCCTTC GGGGGGGGGG 3128472 0.012307 0.000832 0.083181 0.010456
97 6 CTCTATACTG GGGGGGGGGG 2997077 0.011790 0.000797 0.079687 0.010017
98 6 GCAGAAGTCT GGGGGGGGGG 2783276 0.010949 0.000740 0.074002 0.009303
99 6 GATAGGTGAA GGGGGGGGGG 2561031 0.010075 0.000681 0.068093 0.008560
100 6 TAGCGAGGCT GGGGGGGGGG 2413183 0.009493 0.000642 0.064162 0.008066
101 6 TCACCGTGGT GGGGGGGGGG 2104264 0.008278 0.000559 0.055949 0.007033
102 6 TTGCCGTATC GGGGGGGGGG 2068331 0.008137 0.000550 0.054993 0.006913
103 7 ACCAGGAATT GGGGGGGGGG 5604199 0.022596 0.001483 0.148308 0.018731
104 7 AGCCTCTGCT GGGGGGGGGG 5271769 0.021255 0.001395 0.139511 0.017620
105 7 GTGACGCACA GGGGGGGGGG 4296770 0.017324 0.001137 0.113709 0.014361
106 7 AGACGACAGA GGGGGGGGGG 3894373 0.015702 0.001031 0.103060 0.013016
107 7 ACTCGTCCAG GGGGGGGGGG 3883331 0.015657 0.001028 0.102768 0.012979
108 7 GAGTAGTGAC GGGGGGGGGG 3841180 0.015487 0.001017 0.101652 0.012839
109 7 TGGCACTGAT GGGGGGGGGG 3683893 0.014853 0.000975 0.097490 0.012313
110 7 TGAGTGTACA GGGGGGGGGG 3617051 0.014584 0.000957 0.095721 0.012089
111 7 GCATTCCTTC GGGGGGGGGG 3155024 0.012721 0.000835 0.083494 0.010545
112 7 TCTCCGTACT GGGGGGGGGG 2973204 0.011988 0.000787 0.078682 0.009937
113 7 CTCTATACTG GGGGGGGGGG 2957480 0.011924 0.000783 0.078266 0.009885
114 7 GCAGAAGTCT GGGGGGGGGG 2715372 0.010948 0.000719 0.071859 0.009076
115 7 GATAGGTGAA GGGGGGGGGG 2626419 0.010589 0.000695 0.069505 0.008778
116 7 TAGCGAGGCT GGGGGGGGGG 2293063 0.009245 0.000607 0.060683 0.007664
117 7 TCACCGTGGT GGGGGGGGGG 2117939 0.008539 0.000560 0.056049 0.007079
118 7 TTGCCGTATC GGGGGGGGGG 1993122 0.008036 0.000527 0.052745 0.006662
119 7 ATCCAGCATG AGATCTCGGT 1901800 0.007668 0.000503 0.050329 0.006356
120 8 ACCAGGAATT GGGGGGGGGG 5695957 0.022252 0.001509 0.150887 0.019038
121 8 AGCCTCTGCT GGGGGGGGGG 5401826 0.021103 0.001431 0.143096 0.018055
122 8 GTGACGCACA GGGGGGGGGG 4334850 0.016935 0.001148 0.114831 0.014489
123 8 AGACGACAGA GGGGGGGGGG 4005933 0.015650 0.001061 0.106118 0.013389
124 8 ACTCGTCCAG GGGGGGGGGG 3993991 0.015603 0.001058 0.105802 0.013349
125 8 GAGTAGTGAC GGGGGGGGGG 3856942 0.015068 0.001022 0.102171 0.012891
126 8 TGGCACTGAT GGGGGGGGGG 3766465 0.014714 0.000998 0.099774 0.012589
127 8 TGAGTGTACA GGGGGGGGGG 3693635 0.014430 0.000978 0.097845 0.012345
128 8 GCATTCCTTC GGGGGGGGGG 3227519 0.012609 0.000855 0.085498 0.010787
129 8 TCTCCGTACT GGGGGGGGGG 3060307 0.011956 0.000811 0.081068 0.010229
130 8 CTCTATACTG GGGGGGGGGG 3024366 0.011815 0.000801 0.080116 0.010108
131 8 GCAGAAGTCT GGGGGGGGGG 2817806 0.011008 0.000746 0.074644 0.009418
132 8 GATAGGTGAA GGGGGGGGGG 2667562 0.010421 0.000707 0.070664 0.008916
133 8 TAGCGAGGCT GGGGGGGGGG 2333333 0.009116 0.000618 0.061811 0.007799
134 8 TCACCGTGGT GGGGGGGGGG 2168604 0.008472 0.000574 0.057447 0.007248
135 8 TTGCCGTATC GGGGGGGGGG 1955813 0.007641 0.000518 0.051810 0.006537
5. BARCODES WITH HIGH MISMATCH RATES
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Found 4 barcodes with high mismatch rates:
Lane SampleID Index # Reads Mismatch_Rate
273 5 1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2 GCATTCCTTC-GAGCCTGCTT 29720632 21.362682
176 4 1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2 GCATTCCTTC-GAGCCTGCTT 29694497 20.360355
661 6 1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2 GCATTCCTTC-GAGCCTGCTT 30796012 20.068978
564 3 1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2 GCATTCCTTC-GAGCCTGCTT 30230749 20.006941
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FINAL STATUS CHECK
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Overall % Undetermined: 6.92%
Status: WARNING
Reason(s):
Overall undetermined 6.92% >= 5.0%