Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 10758509 total bases: 1624534859 Q20 bases: 1559358413(95.988%) Q30 bases: 1458141108(89.7575%) Read2 before filtering: total reads: 10758509 total bases: 1624534859 Q20 bases: 1566078747(96.4017%) Q30 bases: 1469706284(90.4694%) Read1 after filtering: total reads: 10301720 total bases: 1061053834 Q20 bases: 1055838610(99.5085%) Q30 bases: 1032654062(97.3234%) Read2 after filtering: total reads: 10301720 total bases: 1068811134 Q20 bases: 1058825938(99.0658%) Q30 bases: 1028902955(96.2661%) Filtering result: reads passed filter: 20603440 reads failed due to low quality: 622298 reads failed due to too many N: 3714 reads failed due to too short: 287566 reads with adapter trimmed: 18400958 bases trimmed due to adapters: 1020567641 Duplication rate: 39.6449% Insert size peak (evaluated by paired-end reads): 86 JSON report: 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1.fastp.json HTML report: 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1.fastp.html fastp --in1 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_1.fastq.gz --in2 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_2.fastq.gz --out1 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_1.fastp.fastq.gz --out2 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_2.fastp.fastq.gz --json 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1.fastp.json --html 1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 21 seconds