+ mkdir 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2 + all_qc -bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai 0it [00:00, ?it/s] 106551it [00:00, 1065325.60it/s] 217555it [00:00, 1091585.59it/s] 328842it [00:00, 1101270.84it/s] 441594it [00:00, 1111605.33it/s] 552898it [00:00, 1112095.31it/s] 666250it [00:00, 1119357.04it/s] 778393it [00:00, 1120007.99it/s] 891080it [00:00, 1122168.07it/s] 1003466it [00:00, 1122677.22it/s] 1116744it [00:01, 1125775.63it/s] 1229322it [00:01, 1123362.57it/s] 1341660it [00:01, 1122530.87it/s] 1454873it [00:01, 1125297.37it/s] 1567404it [00:01, 1114669.32it/s] 1678894it [00:01, 1108859.40it/s] 1789799it [00:01, 1102523.97it/s] 1902304it [00:01, 1109207.87it/s] 2014744it [00:01, 1113722.90it/s] 2128155it [00:01, 1119678.89it/s] 2240137it [00:02, 1105231.31it/s] 2350708it [00:02, 1098261.68it/s] 2460570it [00:02, 1094063.70it/s] 2570704it [00:02, 1096203.51it/s] 2683303it [00:02, 1105050.38it/s] 2797375it [00:02, 1115627.22it/s] 2909777it [00:02, 1118114.65it/s] 3021761it [00:02, 1118608.79it/s] 3133773it [00:02, 1119042.40it/s] 3247703it [00:02, 1125093.04it/s] 3361179it [00:03, 1127970.43it/s] 3473981it [00:03, 1123758.33it/s] 3586364it [00:03, 1121995.26it/s] 3698569it [00:03, 1120081.16it/s] 3811316it [00:03, 1122267.15it/s] 3924096it [00:03, 1123899.16it/s] 4036489it [00:03, 1117598.36it/s] 4148258it [00:03, 1105492.25it/s] 4261673it [00:03, 1113968.96it/s] 4374133it [00:03, 1117111.20it/s] 4485869it [00:04, 1103700.29it/s] 4597136it [00:04, 1106342.64it/s] 4709566it [00:04, 1111667.57it/s] 4823273it [00:04, 1119156.13it/s] 4935614it [00:04, 1120408.72it/s] 5047740it [00:04, 1120646.87it/s] 5161404it [00:04, 1125417.15it/s] 5273956it [00:04, 1124190.99it/s] 5386383it [00:04, 1119677.69it/s] 5498360it [00:04, 1068651.10it/s] 5607583it [00:05, 1075450.16it/s] 5719345it [00:05, 1087761.21it/s] 5831358it [00:05, 1097248.96it/s] 5941299it [00:05, 1094688.98it/s] 6053709it [00:05, 1103395.89it/s] 6165963it [00:05, 1109074.74it/s] 6276955it [00:05, 1109045.60it/s] 6389524it [00:05, 1113997.51it/s] 6501679it [00:05, 1116205.59it/s] 6615630it [00:05, 1123162.68it/s] 6728428it [00:06, 1124584.51it/s] 6840903it [00:06, 1123738.31it/s] 6953311it [00:06, 1123820.94it/s] 7066658it [00:06, 1126628.88it/s] 7179327it [00:06, 1125926.44it/s] 7291924it [00:06, 1125567.41it/s] 7404727it [00:06, 1126286.55it/s] 7518710it [00:06, 1130326.37it/s] 7631745it [00:06, 1127651.56it/s] 7744513it [00:06, 1127190.49it/s] 7857234it [00:07, 1125896.66it/s] 7969826it [00:07, 1121884.53it/s] 8082018it [00:07, 1112900.62it/s] 8193324it [00:07, 1105089.68it/s] 8303851it [00:07, 1104455.83it/s] 8415512it [00:07, 1108056.68it/s] 8526329it [00:07, 1104946.54it/s] 8637020it [00:07, 1105511.96it/s] 8747578it [00:07, 1103986.66it/s] 8859965it [00:07, 1109909.73it/s] 8972708it [00:08, 1115132.60it/s] 9086020it [00:08, 1120498.70it/s] 9200276it [00:08, 1127089.04it/s] 9312989it [00:08, 1125865.92it/s] 9425579it [00:08, 1121624.28it/s] 9537747it [00:08, 1121228.15it/s] 9652143it [00:08, 1128007.88it/s] 9765534it [00:08, 1129751.12it/s] 9878843it [00:08, 1130730.70it/s] 9991919it [00:08, 1128445.82it/s] 10104767it [00:09, 1125395.23it/s] 10217310it [00:09, 1119145.07it/s] 10329233it [00:09, 1118178.83it/s] 10442393it [00:09, 1122165.35it/s] 10555668it [00:09, 1125309.01it/s] 10668204it [00:09, 1124437.29it/s] 10780652it [00:09, 1123278.47it/s] 10892983it [00:09, 1109105.57it/s] 11003933it [00:09, 1094252.77it/s] 11114575it [00:09, 1097821.02it/s] 11226211it [00:10, 1103293.76it/s] 11336580it [00:10, 1101987.04it/s] 11447415it [00:10, 1103862.18it/s][W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai 11560741it [00:10, 1112617.00it/s] 11673661it [00:10, 1117554.87it/s] 11785431it [00:10, 1116760.30it/s] 11897118it [00:10, 1108467.77it/s][W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai 12009702it [00:10, 1113597.91it/s] 12121079it [00:10, 1109346.60it/s] 12232106it [00:10, 1109602.54it/s] 12345467it [00:11, 1116752.23it/s] 12457937it [00:11, 1119109.00it/s] 12570097it [00:11, 1119832.66it/s] 12683034it [00:11, 1122670.67it/s] 12795306it [00:11, 1119592.37it/s] 12907603it [00:11, 1120584.09it/s] 13019803it [00:11, 1120988.38it/s] 13132273it [00:11, 1122079.06it/s] 13151019it [00:11, 1115040.04it/s] [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md.bai + merge_qc -folders 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.md -output 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_full_basic_stats.tsv + head -n1 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_full_basic_stats.tsv