[W::hts_idx_load3] The index file is older than the data file: 1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10979416) splicing reads. 227023 splicing junctions. 177148 known splicing junctions. 49875 novel splicing junctions.
sampling 10% (21958832) splicing reads. 268970 splicing junctions. 189945 known splicing junctions. 79025 novel splicing junctions.
sampling 15% (32938249) splicing reads. 298328 splicing junctions. 196897 known splicing junctions. 101431 novel splicing junctions.
sampling 20% (43917665) splicing reads. 321319 splicing junctions. 201705 known splicing junctions. 119614 novel splicing junctions.
sampling 25% (54897082) splicing reads. 340021 splicing junctions. 205167 known splicing junctions. 134854 novel splicing junctions.
sampling 30% (65876498) splicing reads. 355419 splicing junctions. 207835 known splicing junctions. 147584 novel splicing junctions.
sampling 35% (76855914) splicing reads. 368732 splicing junctions. 209955 known splicing junctions. 158777 novel splicing junctions.
sampling 40% (87835331) splicing reads. 379916 splicing junctions. 211601 known splicing junctions. 168315 novel splicing junctions.
sampling 45% (98814747) splicing reads. 389780 splicing junctions. 213067 known splicing junctions. 176713 novel splicing junctions.
sampling 50% (109794164) splicing reads. 398449 splicing junctions. 214241 known splicing junctions. 184208 novel splicing junctions.
sampling 55% (120773580) splicing reads. 405824 splicing junctions. 215210 known splicing junctions. 190614 novel splicing junctions.
sampling 60% (131752996) splicing reads. 412298 splicing junctions. 216006 known splicing junctions. 196292 novel splicing junctions.
sampling 65% (142732413) splicing reads. 417871 splicing junctions. 216685 known splicing junctions. 201186 novel splicing junctions.
sampling 70% (153711829) splicing reads. 422569 splicing junctions. 217277 known splicing junctions. 205292 novel splicing junctions.
sampling 75% (164691246) splicing reads. 426692 splicing junctions. 217781 known splicing junctions. 208911 novel splicing junctions.
sampling 80% (175670662) splicing reads. 430149 splicing junctions. 218225 known splicing junctions. 211924 novel splicing junctions.
sampling 85% (186650078) splicing reads. 432945 splicing junctions. 218541 known splicing junctions. 214404 novel splicing junctions.
sampling 90% (197629495) splicing reads. 435089 splicing junctions. 218792 known splicing junctions. 216297 novel splicing junctions.
sampling 95% (208608911) splicing reads. 436677 splicing junctions. 218990 known splicing junctions. 217687 novel splicing junctions.
sampling 100% (219588328) splicing reads. 437664 splicing junctions. 219125 known splicing junctions. 218539 novel splicing junctions.