Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2b/979a501e2a3d6cd142d1a684a95f19/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/56/27c91f0b684312a4f8644d8dae4475/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/56/27c91f0b684312a4f8644d8dae4475/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/56/27c91f0b684312a4f8644d8dae4475/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/eea51d9f2d48591b2e0cc217c73c43/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2b/979a501e2a3d6cd142d1a684a95f19/.command.run ==> STAGING COMPLETE (7 inputs) 08:35:48 : 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[16380000], rate=22336363.64/min [16390000], rate=22350000.00/min [16400000], rate=22363636.36/min [16410000], rate=22377272.73/min [16420000], rate=22390909.09/min [16430000], rate=22404545.45/min [16440000], rate=22418181.82/min [16450000], rate=22431818.18/min [16460000], rate=22445454.55/min [16470000], rate=22459090.91/min [16480000], rate=22472727.27/min [16490000], rate=22486363.64/min [16500000], rate=22500000.00/min [16510000], rate=22513636.36/min [16520000], rate=22527272.73/min [16530000], rate=22540909.09/min [16540000], rate=22554545.45/min [16550000], rate=22568181.82/min [16560000], rate=22581818.18/min [16570000], rate=22595454.55/min [16580000], rate=22609090.91/min [16590000], rate=22622727.27/min [16600000], rate=22636363.64/min [16610000], rate=22650000.00/min [16620000], rate=22663636.36/min [16630000], rate=22677272.73/min [16640000], rate=22690909.09/min [16650000], rate=22704545.45/min [16660000], rate=22718181.82/min [16670000], rate=22731818.18/min [16680000], rate=22745454.55/min [16690000], rate=22759090.91/min [16700000], rate=22772727.27/min [16710000], rate=22786363.64/min [16720000], rate=22800000.00/min [16730000], rate=22813636.36/min [16740000], rate=22827272.73/min [16750000], rate=22333333.33/min [16760000], rate=22346666.67/min [16770000], rate=22360000.00/min [16780000], rate=22373333.33/min [16790000], rate=22386666.67/min [16800000], rate=22400000.00/min [16810000], rate=22413333.33/min [16820000], rate=22426666.67/min [16830000], rate=22440000.00/min [16840000], rate=22453333.33/min [16850000], rate=22466666.67/min [16860000], rate=22480000.00/min [16870000], rate=22493333.33/min [16880000], rate=22506666.67/min [16890000], rate=22520000.00/min [16900000], rate=22533333.33/min [16910000], rate=22546666.67/min [16920000], rate=22560000.00/min [16930000], rate=22573333.33/min [16940000], rate=22586666.67/min [16950000], rate=22600000.00/min [16960000], rate=22613333.33/min [16970000], rate=22626666.67/min [16980000], rate=22640000.00/min [16990000], rate=22653333.33/min [17000000], rate=22666666.67/min [17010000], rate=22680000.00/min [17020000], rate=22693333.33/min [17030000], rate=22706666.67/min [17040000], rate=22720000.00/min [17050000], rate=22733333.33/min [17060000], rate=22746666.67/min [17070000], rate=22760000.00/min [17080000], rate=22773333.33/min [17090000], rate=22786666.67/min [17100000], rate=22800000.00/min [17110000], rate=22813333.33/min [17120000], rate=22826666.67/min [17130000], rate=22840000.00/min [17140000], rate=22853333.33/min [17150000], rate=22866666.67/min [17160000], rate=22880000.00/min [17170000], rate=22893333.33/min [17180000], rate=22408695.65/min [17190000], rate=22421739.13/min [17200000], rate=22434782.61/min 08:36:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 4037 cancer introns 08:36:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 08:36:42 : INFO : Running: touch /tmp/nxf.clYRywBqnU/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok 08:36:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 08:36:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 08:36:42 : INFO : Running: touch /tmp/nxf.clYRywBqnU/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok 08:36:42 : INFO : -found 1553 cancer introns 08:36:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:36:43 : INFO : Creating the BED File. 08:36:46 : INFO : Saving Bed File as 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:36:46 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:36:46 : INFO : Running: touch /tmp/nxf.clYRywBqnU/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok 08:36:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2 [W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai 08:52:35 : INFO : Execution Time = 15.83 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2 08:52:35 : INFO : Running: touch /tmp/nxf.clYRywBqnU/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok 08:52:36 : INFO : Running: samtools sort -o 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 08:52:36 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 08:52:36 : INFO : Running: touch /tmp/nxf.clYRywBqnU/1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok 08:52:36 : INFO : Running: samtools sort -o 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam