Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/ad00d91b871c77c26e40f9a6b6b84d/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b0/819b9e5f146b59f037c0b19d0b71e9/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/ad00d91b871c77c26e40f9a6b6b84d/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b0/819b9e5f146b59f037c0b19d0b71e9/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/ad00d91b871c77c26e40f9a6b6b84d/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f7/9b2a39216732e94a63b9c756b6e117/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai ==> STAGING COMPLETE (7 inputs) 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/usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 3911 cancer introns 08:45:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 08:45:42 : INFO : Running: touch /tmp/nxf.0NKEDnNtTM/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok 08:45:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 08:45:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 08:45:42 : INFO : Running: touch /tmp/nxf.0NKEDnNtTM/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok 08:45:42 : INFO : -found 2064 cancer introns 08:45:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:45:42 : INFO : Creating the BED File. 08:45:47 : INFO : Saving Bed File as 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:45:47 : INFO : Execution Time = 0.08 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 08:45:47 : INFO : Running: touch /tmp/nxf.0NKEDnNtTM/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok 08:45:47 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2 [W::hts_idx_load3] The index file is older than the data file: 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai 09:04:49 : INFO : Execution Time = 19.03 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2 09:04:49 : INFO : Running: touch /tmp/nxf.0NKEDnNtTM/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok 09:04:49 : INFO : Running: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 09:04:49 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 09:04:49 : INFO : Running: touch /tmp/nxf.0NKEDnNtTM/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok 09:04:49 : INFO : Running: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam