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rate=26464864.86/min [16330000], rate=26481081.08/min [16340000], rate=26497297.30/min [16350000], rate=26513513.51/min [16360000], rate=26529729.73/min [16370000], rate=26545945.95/min [16380000], rate=26562162.16/min [16390000], rate=26578378.38/min [16400000], rate=26594594.59/min [16410000], rate=26610810.81/min [16420000], rate=26627027.03/min [16430000], rate=25942105.26/min [16440000], rate=25957894.74/min [16450000], rate=25973684.21/min [16460000], rate=25989473.68/min [16470000], rate=26005263.16/min [16480000], rate=26021052.63/min [16490000], rate=26036842.11/min [16500000], rate=26052631.58/min 09:12:59 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 3911 cancer introns 09:13:00 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 09:13:00 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok 09:13:00 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 09:13:00 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 09:13:00 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok 09:13:00 : INFO : -found 2064 cancer introns 09:13:00 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 09:13:01 : INFO : Creating the BED File. 09:13:05 : INFO : Saving Bed File as 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 09:13:05 : INFO : Execution Time = 0.07 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 09:13:05 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok 09:13:05 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2 [W::hts_idx_load3] The index file is older than the data file: 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai 09:29:33 : INFO : Execution Time = 16.47 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2 09:29:33 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok 09:29:33 : INFO : Running: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 09:29:33 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 09:29:33 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok 09:29:33 : INFO : Running: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam [bam_sort_core] merging from 101 files and 1 in-memory blocks... 09:49:46 : INFO : Execution Time = 20.21 minutes. CMD: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam 09:49:46 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_gene_reads.ok 09:49:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 09:56:34 : INFO : Execution Time = 6.80 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 09:56:34 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp.ok 09:56:34 : INFO : Running: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp [bam_sort_core] merging from 2 files and 1 in-memory blocks... 09:57:06 : INFO : Execution Time = 0.52 minutes. CMD: samtools sort -o 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp 09:57:06 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmpsorted.ok 09:57:06 : INFO : Running: samtools index 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 09:57:09 : INFO : Execution Time = 0.06 minutes. CMD: samtools index 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 09:57:09 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.indexed.ok 09:57:09 : INFO : Running: samtools index 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 09:57:09 : INFO : Execution Time = 0.00 minutes. CMD: samtools index 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 09:57:09 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam.indexed.ok 09:57:09 : INFO : Running: create_report 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.ctat-splicing.igv.html --track-config 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2' 10:01:43 : INFO : Execution Time = 4.57 minutes. CMD: create_report 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.ctat-splicing.igv.html --track-config 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2' 10:01:43 : INFO : Running: touch /tmp/nxf.bNuIOA710T/1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/igv_create_html.ok 10:01:43 : INFO : done.