File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/980931caf60bd8bea6431901aa9289/.command.log
Size
49.8 KB
Attempt
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/980931caf60bd8bea6431901aa9289/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d3/ff608cbe6741216a618646025b994a/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/93/ef39f8d424fb692db6930ec8066710/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Chimeric.out.junction
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/93/ef39f8d424fb692db6930ec8066710/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.SJ.out.tab
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/93/ef39f8d424fb692db6930ec8066710/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/980931caf60bd8bea6431901aa9289/.command.run
==> STAGING COMPLETE (7 inputs)

08:35:53 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes

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[14110000], rate=21165000.00/min 08:36:41 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 4833 cancer introns
08:36:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 
08:36:42 : INFO : Running: touch /tmp/nxf.AW4ndUsRHR/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok
08:36:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5  > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 
08:36:42 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5  > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 
08:36:42 : INFO : Running: touch /tmp/nxf.AW4ndUsRHR/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok
08:36:42 : INFO : -found 1448 cancer introns
08:36:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns  --cancer_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 
08:36:42 : INFO :  Creating the BED File.
08:36:45 : INFO : Saving Bed File as 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed
08:36:45 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns  --cancer_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 
08:36:45 : INFO : Running: touch /tmp/nxf.AW4ndUsRHR/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok
08:36:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed  --bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam  --output_prefix 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2 
[W::hts_idx_load3] The index file is older than the data file: 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
08:47:06 : INFO : Execution Time = 10.33 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed  --bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam  --output_prefix 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2 
08:47:06 : INFO : Running: touch /tmp/nxf.AW4ndUsRHR/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok
08:47:06 : INFO : Running: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam
08:47:06 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam
08:47:06 : INFO : Running: touch /tmp/nxf.AW4ndUsRHR/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok
08:47:06 : INFO : Running: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam
[bam_sort_core] merging from 58 files and 1 in-memory blocks...