Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/25/cd1c3ff6b83aa2151ace6f643dbdc0/1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.tsv
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/11/78e1092852607f2d3c89d24e19dbfd/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/11/78e1092852607f2d3c89d24e19dbfd/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-fe242a7f-c5b9-4d3f-88fa-f3e739280d3a/f8/f9224e55c50c449a43c8a740b583b3/starfusion_filter_config.yaml
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/blacklist_breakpoint_database_2026-04-27.tsv
==> STAGING COMPLETE (6 inputs)

=== DEBUGGING INFORMATION ===
Current working directory: /tmp/nxf.MrOQXMy3Q2
Input files:
  - starfusion_tsv: 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.tsv
  - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  - filter_config: starfusion_filter_config.yaml
  - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv

=== PYTHON INFORMATION ===
/usr/local/bin/python
Python 3.11.14
/usr/local/bin/python3
Python 3.11.14

=== SCRIPT LOCATION ===
Checking for filter_starfusion_main.py:
/tmp/nxf.MrOQXMy3Q2/nextflow-bin/filter_starfusion_main.py
filter_starfusion_main.py not in current directory
bin/filter_starfusion_main.py not found
bin/ directory does not exist

=== ENVIRONMENT PATH ===
/tmp/nxf.MrOQXMy3Q2/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin

=== CURRENT DIRECTORY CONTENTS ===
total 64332
drwx------. 3 1000 root     4096 Jun  8 11:36 .
drwxrwxrwx. 4 root root       86 Jun  8 11:36 ..
-rw-r--r--. 1 1000 root        0 Jun  8 11:36 .command.err
-rw-r--r--. 1 1000 root      886 Jun  8 11:36 .command.out
-rw-r--r--. 1 1000 root    14597 Jun  8 11:36 .command.run
-rw-r--r--. 1 1000 root     3248 Jun  8 11:36 .command.sh
-rw-r--r--. 1 1000 root        0 Jun  8 11:36 .command.trace
-rw-r--r--. 1 1000 root   336018 Jun  8 11:36 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.tsv
-rw-r--r--. 1 1000 root 10714998 Jun  8 11:36 blacklist_breakpoint_database_2026-04-27.tsv
-rw-r--r--. 1 1000 root 54779787 Jun  8 11:36 blacklist_hg38_GRCh38_v2.4.0.tsv.gz
drwxr-xr-x. 3 1000 root     4096 Jun  8 11:36 nextflow-bin
-rw-r--r--. 1 1000 root     4128 Jun  8 11:36 starfusion_filter_config.yaml

=== PROJECT DIRECTORY INFO ===
NXF_PROJECT_DIR: not set
baseDir: not set

=== Running filter script ===

================================================================================
STEP 1: LOADING STAR-FUSION PREDICTIONS
================================================================================
Loading STAR-Fusion predictions from: 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.tsv
  ✓ Loaded: 431 fusions

================================================================================
STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT
================================================================================
✓ Converted to breakpoint_ID format: 431 valid, 0 invalid

================================================================================
STEP 3: FLAGGING WITH BLACKLIST
================================================================================
Loading blacklist (this may take a while)...
Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Loaded 9381 single rules and 16721785 pair rules
  Indexed blacklist (bin_size=100000)
Flagging fusions with blacklist...
✓ Flagged 125 of 431 fusions as blacklisted

================================================================================
STEP 3b: AIH CURATED BREAKPOINT BLACKLIST
================================================================================
Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv
  Qualified breakpoint keys (orientations): 1378
  AIH curated blacklist: matched 75 fusion rows (breakpoint_ID in qualified set)

================================================================================
STEP 4: APPLYING YAML-BASED FILTER
================================================================================
✓ Filter initialized from: starfusion_filter_config.yaml
Applying filter criteria (adding CALL column)...
✓ Filter applied: 22 pass (CALL=1), 409 fail (CALL=0)
  Total fusions: 431 (all rows retained)
  Added FILTER column: PASS=22, FAIL=409

✓ Verified: All 147 blacklisted fusions have CALL=0

================================================================================
STEP 5: GENERATING SUMMARY STATISTICS
================================================================================

================================================================================
FILTERING SUMMARY
================================================================================

Total fusions: 431
  Pass (CALL=1): 22 (5.1%)
  Fail (CALL=0): 409 (94.9%)

Blacklist Flagging:
  Total blacklisted: 147
  ✓ All 147 blacklisted fusions have CALL=0

Failure Reasons (top 10):
  Read count too low: 1 < 8: 161
  Excluded SpliceType: INCL_NON_REF_SPLICE: 76
  Blacklisted: 72
  Blacklisted; aih_curated_blacklist score(3): 53
  aih_curated_blacklist score(3): 20
  Excluded annotation pattern: HGNC_GENEFAM: 7
  Read count too low: 2 < 8: 6
  Read count too low: 4 < 8: 4
  Read count too low: 7 < 8: 2
  Read count too low: 6 < 8: 2

Breakdown by SpliceType:
  INCL_NON_REF_SPLICE: 4 pass, 230 fail (total: 234)
  ONLY_REF_SPLICE: 18 pass, 179 fail (total: 197)

Read Count Statistics:
  Fusions with < 3 total reads: 294
  Of these, 294 have CALL=0

================================================================================
STEP 6: EXPORTING RESULTS
================================================================================
✓ Results exported to: 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.filtered.tsv
  Total fusions: 431
  CALL=1 (pass): 22
  CALL=0 (fail): 409
✓ Passing fusions (CALL=1) exported to: 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv
✓ Summary exported to: 1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2.starfusion.starfusion.fusion_predictions.filter_summary.txt

================================================================================
✓ FILTERING COMPLETE
================================================================================