#!/bin/bash -euo pipefail
python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \
--SJ_tab_file NTC_0001_0001_A23T55JLT4_1.SJ.out.tab \
--chimJ_file NTC_0001_0001_A23T55JLT4_1.Chimeric.out.junction \
--bam_file NTC_0001_0001_A23T55JLT4_1.Aligned.sortedByCoord.out.bam \
--output_prefix NTC_0001_0001_A23T55JLT4_1 \
--ctat_genome_lib ctat_genome_lib_build_dir \
--vis --sample_name NTC_0001_0001_A23T55JLT4_1
# Sort output files to ensure consistent ordering across runs while preserving headers
if [ -f NTC_0001_0001_A23T55JLT4_1.cancer.introns ]; then
# Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron)
head -n 1 NTC_0001_0001_A23T55JLT4_1.cancer.introns > NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp
tail -n +2 NTC_0001_0001_A23T55JLT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp
mv NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp NTC_0001_0001_A23T55JLT4_1.cancer.introns
fi
cat <<-END_VERSIONS > versions.yml
"NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS":
ctat-splicing: 0.0.3
END_VERSIONS