#!/bin/bash -euo pipefail python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \ --SJ_tab_file NTC_0001_0001_A23T55JLT4_1.SJ.out.tab \ --chimJ_file NTC_0001_0001_A23T55JLT4_1.Chimeric.out.junction \ --bam_file NTC_0001_0001_A23T55JLT4_1.Aligned.sortedByCoord.out.bam \ --output_prefix NTC_0001_0001_A23T55JLT4_1 \ --ctat_genome_lib ctat_genome_lib_build_dir \ --vis --sample_name NTC_0001_0001_A23T55JLT4_1 # Sort output files to ensure consistent ordering across runs while preserving headers if [ -f NTC_0001_0001_A23T55JLT4_1.cancer.introns ]; then # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron) head -n 1 NTC_0001_0001_A23T55JLT4_1.cancer.introns > NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp tail -n +2 NTC_0001_0001_A23T55JLT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp mv NTC_0001_0001_A23T55JLT4_1.cancer.introns.tmp NTC_0001_0001_A23T55JLT4_1.cancer.introns fi cat <<-END_VERSIONS > versions.yml "NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS": ctat-splicing: 0.0.3 END_VERSIONS