+ mkdir 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1 + all_qc -bam 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai 0it [00:00, ?it/s] 130625it [00:00, 1306132.91it/s] 273140it [00:00, 1376090.98it/s] 426030it [00:00, 1445828.45it/s] 588563it [00:00, 1516634.37it/s] 757655it [00:00, 1579450.65it/s] 927904it [00:00, 1621252.56it/s] 1098126it [00:00, 1647529.51it/s] 1269717it [00:00, 1669273.95it/s] 1440311it [00:00, 1680712.05it/s] 1612918it [00:01, 1694692.09it/s] 1785013it [00:01, 1702637.73it/s] 1957830it [00:01, 1710241.10it/s] 2128854it [00:01, 1710049.85it/s] 2301227it [00:01, 1714157.06it/s] 2473729it [00:01, 1717411.83it/s] 2645471it [00:01, 1708802.02it/s] 2816362it [00:01, 1701932.81it/s] 2988687it [00:01, 1708274.31it/s] 3159526it [00:01, 1679524.95it/s] 3330269it [00:02, 1687758.68it/s] 3499132it [00:02, 1683036.93it/s] 3667863it [00:02, 1684282.96it/s] 3842246it [00:02, 1701941.32it/s] 4019728it [00:02, 1723667.85it/s] 4196783it [00:02, 1737659.80it/s] 4370581it [00:02, 1736297.62it/s] 4544233it [00:02, 1729342.69it/s] 4717188it [00:02, 1717587.59it/s] 4888975it [00:02, 1697194.04it/s] 5062167it [00:03, 1707442.98it/s] 5234492it [00:03, 1712117.61it/s] 5405748it [00:03, 1711956.82it/s] 5579724it [00:03, 1720234.00it/s] 5752550it [00:03, 1722608.19it/s] 5924830it [00:03, 1716216.38it/s] 6096469it [00:03, 1714338.51it/s] 6267915it [00:03, 1708735.76it/s] 6441429it [00:03, 1716580.23it/s] 6613099it [00:03, 1705501.12it/s] 6788811it [00:04, 1720858.25it/s] 6962855it [00:04, 1726684.75it/s] 7135544it [00:04, 1721898.58it/s] 7307750it [00:04, 1720202.89it/s] 7480432it [00:04, 1722163.83it/s] 7655257it [00:04, 1729848.46it/s] 7828250it [00:04, 1722112.17it/s] 8000474it [00:04, 1713581.66it/s] 8172416it [00:04, 1715303.77it/s] 8343958it [00:04, 1713707.77it/s] 8515337it [00:05, 1706567.88it/s] 8686004it [00:05, 1704330.96it/s] 8857961it [00:05, 1708863.69it/s] 9028854it [00:05, 1694399.45it/s] 9202682it [00:05, 1707437.15it/s] 9373457it [00:05, 1701605.87it/s] 9544514it [00:05, 1704261.04it/s] 9718000it [00:05, 1713381.96it/s] 9889355it [00:05, 1710743.32it/s] 10060921it [00:05, 1712055.20it/s] 10232136it [00:06, 1703680.48it/s] 10402518it [00:06, 1684902.65it/s] 10574003it [00:06, 1693765.09it/s] 10743423it [00:06, 1684998.58it/s] 10912838it [00:06, 1687703.50it/s] 11086474it [00:06, 1702190.19it/s] 11260387it [00:06, 1713158.53it/s] 11434037it [00:06, 1720124.12it/s] 11607332it [00:06, 1723948.88it/s] 11779742it [00:06, 1721807.24it/s] 11951934it [00:07, 1716407.69it/s] 12124661it [00:07, 1719585.24it/s] 12298431it [00:07, 1724992.71it/s] 12472123it [00:07, 1728552.61it/s] 12644984it [00:07, 1720774.48it/s] 12817073it [00:07, 1715455.78it/s] 12988629it [00:07, 1711858.12it/s] 13160435it [00:07, 1713680.62it/s] 13332084it [00:07, 1714503.85it/s] 13503741it [00:07, 1714925.66it/s] 13675237it [00:08, 1713946.46it/s] 13846634it [00:08, 1700215.72it/s] 14020333it [00:08, 1711156.56it/s] 14192101it [00:08, 1713087.67it/s] 14363428it [00:08, 1707471.80it/s] 14534610it [00:08, 1708634.42it/s] 14705902it [00:08, 1709901.68it/s] 14880047it [00:08, 1719329.84it/s] 15051988it [00:08, 1713211.78it/s] 15224156it [00:08, 1715727.57it/s] 15395737it [00:09, 1714562.54it/s] 15567199it [00:09, 1698716.80it/s] 15737105it [00:09, 1690327.59it/s] 15906165it [00:09, 1682934.43it/s] 16074478it [00:09, 1680201.69it/s] 16242511it [00:09, 1679000.01it/s] 16410419it [00:09, 1668117.15it/s] 16577247it [00:09, 1666560.29it/s] 16745667it [00:09, 1671799.95it/s] 16913909it [00:09, 1674924.67it/s] 17081411it [00:10, 1657481.37it/s][W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai 17250552it [00:10, 1667544.16it/s] 17417346it [00:10, 1649454.69it/s] 17582353it [00:10, 1629253.42it/s] 17753557it [00:10, 1653643.50it/s] 17925291it [00:10, 1672502.78it/s] 18093873it [00:10, 1676355.91it/s] 18261576it [00:10, 1671835.38it/s] 18433429it [00:10, 1685607.21it/s] 18608599it [00:10, 1705248.10it/s] 18781730it [00:11, 1712988.82it/s] 18954795it [00:11, 1718259.93it/s] 19127823it [00:11, 1721844.81it/s] 19303382it [00:11, 1731940.37it/s] 19476588it [00:11, 1712288.71it/s] 19647875it [00:11, 1702657.59it/s][W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai 19820955it [00:11, 1710998.32it/s] 19993591it [00:11, 1715557.91it/s] 20165264it [00:11, 1715869.22it/s] 20338148it [00:11, 1719730.27it/s] 20510209it [00:12, 1719976.86it/s] 20682219it [00:12, 1707113.09it/s] 20852959it [00:12, 1695161.11it/s] 21022507it [00:12, 1680214.34it/s] 21192815it [00:12, 1686870.85it/s] 21361538it [00:12, 1685123.26it/s] 21532889it [00:12, 1693562.15it/s] 21704799it [00:12, 1701135.94it/s] 21875296it [00:12, 1702103.04it/s] 22045988it [00:12, 1703527.91it/s] 22219045it [00:13, 1711608.15it/s] 22390215it [00:13, 1708993.07it/s] 22561617it [00:13, 1710482.80it/s] 22732671it [00:13, 1708661.22it/s] 22903965it [00:13, 1709926.04it/s] 23076345it [00:13, 1714066.53it/s] 23247755it [00:13, 1696991.90it/s] 23418815it [00:13, 1701028.11it/s] 23588949it [00:13, 1699917.11it/s] 23763033it [00:13, 1712125.40it/s] 23934266it [00:14, 1703122.94it/s] 24104601it [00:14, 1701007.80it/s] 24274717it [00:14, 1692101.05it/s] 24446262it [00:14, 1699040.40it/s] 24460234it [00:14, 1698090.47it/s] [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md.bai + merge_qc -folders 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.md -output 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_full_basic_stats.tsv + head -n1 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_full_basic_stats.tsv