Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0b/ca4d20e41802bc8c33ea65523f74cb/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8d/887cbbdd6123c56d219d52c57d79f6/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7a/55d4990929a49b1df9a5c04c10e423/1173_C31_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8d/887cbbdd6123c56d219d52c57d79f6/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/b74a88b22553b91638147acef41436/1173_C31_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: 1173_C31_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (4533554) splicing reads. 184581 splicing junctions. 159145 known splicing junctions. 25436 novel splicing junctions.
sampling 10% (9067109) splicing reads. 215281 splicing junctions. 172703 known splicing junctions. 42578 novel splicing junctions.
sampling 15% (13600664) splicing reads. 236688 splicing junctions. 180293 known splicing junctions. 56395 novel splicing junctions.
sampling 20% (18134219) splicing reads. 253380 splicing junctions. 185336 known splicing junctions. 68044 novel splicing junctions.
sampling 25% (22667774) splicing reads. 267133 splicing junctions. 189026 known splicing junctions. 78107 novel splicing junctions.
sampling 30% (27201329) splicing reads. 278721 splicing junctions. 191902 known splicing junctions. 86819 novel splicing junctions.
sampling 35% (31734884) splicing reads. 288793 splicing junctions. 194207 known splicing junctions. 94586 novel splicing junctions.
sampling 40% (36268439) splicing reads. 297504 splicing junctions. 196155 known splicing junctions. 101349 novel splicing junctions.
sampling 45% (40801994) splicing reads. 305238 splicing junctions. 197780 known splicing junctions. 107458 novel splicing junctions.
sampling 50% (45335549) splicing reads. 312008 splicing junctions. 199202 known splicing junctions. 112806 novel splicing junctions.
sampling 55% (49869103) splicing reads. 317915 splicing junctions. 200349 known splicing junctions. 117566 novel splicing junctions.
sampling 60% (54402658) splicing reads. 323164 splicing junctions. 201367 known splicing junctions. 121797 novel splicing junctions.
sampling 65% (58936213) splicing reads. 327734 splicing junctions. 202234 known splicing junctions. 125500 novel splicing junctions.
sampling 70% (63469768) splicing reads. 331674 splicing junctions. 202965 known splicing junctions. 128709 novel splicing junctions.
sampling 75% (68003323) splicing reads. 335151 splicing junctions. 203648 known splicing junctions. 131503 novel splicing junctions.
sampling 80% (72536878) splicing reads. 337950 splicing junctions. 204129 known splicing junctions. 133821 novel splicing junctions.
sampling 85% (77070433) splicing reads. 340363 splicing junctions. 204568 known splicing junctions. 135795 novel splicing junctions.
sampling 90% (81603988) splicing reads. 342222 splicing junctions. 204892 known splicing junctions. 137330 novel splicing junctions.
sampling 95% (86137543) splicing reads. 343627 splicing junctions. 205128 known splicing junctions. 138499 novel splicing junctions.
sampling 100% (90671098) splicing reads. 344685 splicing junctions. 205320 known splicing junctions. 139365 novel splicing junctions.
null device 
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