Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/453058e4074b8ab3b3e5da1bf4115a/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/453058e4074b8ab3b3e5da1bf4115a/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_2.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/23/5caf471a2a77592746b544b2666166/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/23/5caf471a2a77592746b544b2666166/.command.run ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz input2/1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2 SM:1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 08 07:33:57 ..... started STAR run Jun 08 07:33:57 ..... loading genome Jun 08 07:34:27 ..... processing annotations GTF Jun 08 07:34:36 ..... inserting junctions into the genome indices Jun 08 07:35:57 ..... started mapping Jun 08 08:10:47 ..... finished mapping Jun 08 08:10:51 ..... started sorting BAM Jun 08 08:14:09 ..... finished successfully