[W::hts_idx_load3] The index file is older than the data file: 1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12035663) splicing reads. 223097 splicing junctions. 174851 known splicing junctions. 48246 novel splicing junctions.
sampling 10% (24071327) splicing reads. 260403 splicing junctions. 186198 known splicing junctions. 74205 novel splicing junctions.
sampling 15% (36106991) splicing reads. 284680 splicing junctions. 192203 known splicing junctions. 92477 novel splicing junctions.
sampling 20% (48142655) splicing reads. 302341 splicing junctions. 196030 known splicing junctions. 106311 novel splicing junctions.
sampling 25% (60178319) splicing reads. 316016 splicing junctions. 198759 known splicing junctions. 117257 novel splicing junctions.
sampling 30% (72213983) splicing reads. 326821 splicing junctions. 200772 known splicing junctions. 126049 novel splicing junctions.
sampling 35% (84249647) splicing reads. 335476 splicing junctions. 202287 known splicing junctions. 133189 novel splicing junctions.
sampling 40% (96285311) splicing reads. 342758 splicing junctions. 203518 known splicing junctions. 139240 novel splicing junctions.
sampling 45% (108320975) splicing reads. 348741 splicing junctions. 204467 known splicing junctions. 144274 novel splicing junctions.
sampling 50% (120356639) splicing reads. 354052 splicing junctions. 205297 known splicing junctions. 148755 novel splicing junctions.
sampling 55% (132392302) splicing reads. 358427 splicing junctions. 205953 known splicing junctions. 152474 novel splicing junctions.
sampling 60% (144427966) splicing reads. 362152 splicing junctions. 206509 known splicing junctions. 155643 novel splicing junctions.
sampling 65% (156463630) splicing reads. 365286 splicing junctions. 206993 known splicing junctions. 158293 novel splicing junctions.
sampling 70% (168499294) splicing reads. 367861 splicing junctions. 207358 known splicing junctions. 160503 novel splicing junctions.
sampling 75% (180534958) splicing reads. 369899 splicing junctions. 207639 known splicing junctions. 162260 novel splicing junctions.
sampling 80% (192570622) splicing reads. 371595 splicing junctions. 207890 known splicing junctions. 163705 novel splicing junctions.
sampling 85% (204606286) splicing reads. 372925 splicing junctions. 208083 known splicing junctions. 164842 novel splicing junctions.
sampling 90% (216641950) splicing reads. 373933 splicing junctions. 208241 known splicing junctions. 165692 novel splicing junctions.
sampling 95% (228677614) splicing reads. 374656 splicing junctions. 208357 known splicing junctions. 166299 novel splicing junctions.
sampling 100% (240713278) splicing reads. 375029 splicing junctions. 208419 known splicing junctions. 166610 novel splicing junctions.