#!/bin/bash -euo pipefail python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \ --SJ_tab_file 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.SJ.out.tab \ --chimJ_file 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.Chimeric.out.junction \ --bam_file 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam \ --output_prefix 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2 \ --ctat_genome_lib ctat_genome_lib_build_dir \ --vis --sample_name 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2 # Sort output files to ensure consistent ordering across runs while preserving headers if [ -f 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns ]; then # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron) head -n 1 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns > 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.tmp tail -n +2 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.tmp mv 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.tmp 1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns fi cat <<-END_VERSIONS > versions.yml "NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS": ctat-splicing: 0.0.3 END_VERSIONS