[W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9106626) splicing reads. 216958 splicing junctions. 171630 known splicing junctions. 45328 novel splicing junctions.
sampling 10% (18213252) splicing reads. 259356 splicing junctions. 184932 known splicing junctions. 74424 novel splicing junctions.
sampling 15% (27319878) splicing reads. 289383 splicing junctions. 192091 known splicing junctions. 97292 novel splicing junctions.
sampling 20% (36426504) splicing reads. 313399 splicing junctions. 197002 known splicing junctions. 116397 novel splicing junctions.
sampling 25% (45533130) splicing reads. 333407 splicing junctions. 200574 known splicing junctions. 132833 novel splicing junctions.
sampling 30% (54639756) splicing reads. 350429 splicing junctions. 203438 known splicing junctions. 146991 novel splicing junctions.
sampling 35% (63746382) splicing reads. 365219 splicing junctions. 205695 known splicing junctions. 159524 novel splicing junctions.
sampling 40% (72853008) splicing reads. 377843 splicing junctions. 207449 known splicing junctions. 170394 novel splicing junctions.
sampling 45% (81959634) splicing reads. 389103 splicing junctions. 208905 known splicing junctions. 180198 novel splicing junctions.
sampling 50% (91066261) splicing reads. 398826 splicing junctions. 210153 known splicing junctions. 188673 novel splicing junctions.
sampling 55% (100172887) splicing reads. 407526 splicing junctions. 211229 known splicing junctions. 196297 novel splicing junctions.
sampling 60% (109279513) splicing reads. 415186 splicing junctions. 212115 known splicing junctions. 203071 novel splicing junctions.
sampling 65% (118386139) splicing reads. 421862 splicing junctions. 212946 known splicing junctions. 208916 novel splicing junctions.
sampling 70% (127492765) splicing reads. 427678 splicing junctions. 213588 known splicing junctions. 214090 novel splicing junctions.
sampling 75% (136599391) splicing reads. 432613 splicing junctions. 214163 known splicing junctions. 218450 novel splicing junctions.
sampling 80% (145706017) splicing reads. 436900 splicing junctions. 214690 known splicing junctions. 222210 novel splicing junctions.
sampling 85% (154812643) splicing reads. 440360 splicing junctions. 215025 known splicing junctions. 225335 novel splicing junctions.
sampling 90% (163919269) splicing reads. 443131 splicing junctions. 215351 known splicing junctions. 227780 novel splicing junctions.
sampling 95% (173025895) splicing reads. 445236 splicing junctions. 215580 known splicing junctions. 229656 novel splicing junctions.
sampling 100% (182132522) splicing reads. 446704 splicing junctions. 215760 known splicing junctions. 230944 novel splicing junctions.