[W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13623903) splicing reads. 223944 splicing junctions. 168073 known splicing junctions. 55871 novel splicing junctions.
sampling 10% (27247806) splicing reads. 267076 splicing junctions. 180280 known splicing junctions. 86796 novel splicing junctions.
sampling 15% (40871709) splicing reads. 295103 splicing junctions. 186496 known splicing junctions. 108607 novel splicing junctions.
sampling 20% (54495612) splicing reads. 315411 splicing junctions. 190307 known splicing junctions. 125104 novel splicing junctions.
sampling 25% (68119515) splicing reads. 331127 splicing junctions. 192981 known splicing junctions. 138146 novel splicing junctions.
sampling 30% (81743418) splicing reads. 343622 splicing junctions. 194912 known splicing junctions. 148710 novel splicing junctions.
sampling 35% (95367321) splicing reads. 353920 splicing junctions. 196470 known splicing junctions. 157450 novel splicing junctions.
sampling 40% (108991224) splicing reads. 362206 splicing junctions. 197678 known splicing junctions. 164528 novel splicing junctions.
sampling 45% (122615127) splicing reads. 369318 splicing junctions. 198647 known splicing junctions. 170671 novel splicing junctions.
sampling 50% (136239030) splicing reads. 375298 splicing junctions. 199444 known splicing junctions. 175854 novel splicing junctions.
sampling 55% (149862933) splicing reads. 380358 splicing junctions. 200089 known splicing junctions. 180269 novel splicing junctions.
sampling 60% (163486836) splicing reads. 384615 splicing junctions. 200649 known splicing junctions. 183966 novel splicing junctions.
sampling 65% (177110739) splicing reads. 388181 splicing junctions. 201099 known splicing junctions. 187082 novel splicing junctions.
sampling 70% (190734642) splicing reads. 391111 splicing junctions. 201458 known splicing junctions. 189653 novel splicing junctions.
sampling 75% (204358545) splicing reads. 393562 splicing junctions. 201746 known splicing junctions. 191816 novel splicing junctions.
sampling 80% (217982448) splicing reads. 395470 splicing junctions. 201971 known splicing junctions. 193499 novel splicing junctions.
sampling 85% (231606351) splicing reads. 396929 splicing junctions. 202163 known splicing junctions. 194766 novel splicing junctions.
sampling 90% (245230254) splicing reads. 398068 splicing junctions. 202298 known splicing junctions. 195770 novel splicing junctions.
sampling 95% (258854157) splicing reads. 398814 splicing junctions. 202386 known splicing junctions. 196428 novel splicing junctions.
sampling 100% (272478061) splicing reads. 399198 splicing junctions. 202434 known splicing junctions. 196764 novel splicing junctions.