[W::hts_idx_load3] The index file is older than the data file: 1173_FM_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12213807) splicing reads. 216938 splicing junctions. 169155 known splicing junctions. 47783 novel splicing junctions.
sampling 10% (24427614) splicing reads. 256906 splicing junctions. 181739 known splicing junctions. 75167 novel splicing junctions.
sampling 15% (36641421) splicing reads. 283475 splicing junctions. 188211 known splicing junctions. 95264 novel splicing junctions.
sampling 20% (48855228) splicing reads. 303421 splicing junctions. 192391 known splicing junctions. 111030 novel splicing junctions.
sampling 25% (61069035) splicing reads. 318791 splicing junctions. 195388 known splicing junctions. 123403 novel splicing junctions.
sampling 30% (73282842) splicing reads. 331277 splicing junctions. 197601 known splicing junctions. 133676 novel splicing junctions.
sampling 35% (85496650) splicing reads. 341816 splicing junctions. 199387 known splicing junctions. 142429 novel splicing junctions.
sampling 40% (97710457) splicing reads. 350705 splicing junctions. 200782 known splicing junctions. 149923 novel splicing junctions.
sampling 45% (109924264) splicing reads. 358164 splicing junctions. 201860 known splicing junctions. 156304 novel splicing junctions.
sampling 50% (122138071) splicing reads. 364569 splicing junctions. 202813 known splicing junctions. 161756 novel splicing junctions.
sampling 55% (134351878) splicing reads. 369786 splicing junctions. 203525 known splicing junctions. 166261 novel splicing junctions.
sampling 60% (146565685) splicing reads. 374321 splicing junctions. 204132 known splicing junctions. 170189 novel splicing junctions.
sampling 65% (158779492) splicing reads. 378258 splicing junctions. 204697 known splicing junctions. 173561 novel splicing junctions.
sampling 70% (170993300) splicing reads. 381547 splicing junctions. 205118 known splicing junctions. 176429 novel splicing junctions.
sampling 75% (183207107) splicing reads. 384188 splicing junctions. 205453 known splicing junctions. 178735 novel splicing junctions.
sampling 80% (195420914) splicing reads. 386322 splicing junctions. 205744 known splicing junctions. 180578 novel splicing junctions.
sampling 85% (207634721) splicing reads. 388012 splicing junctions. 205953 known splicing junctions. 182059 novel splicing junctions.
sampling 90% (219848528) splicing reads. 389364 splicing junctions. 206118 known splicing junctions. 183246 novel splicing junctions.
sampling 95% (232062335) splicing reads. 390192 splicing junctions. 206240 known splicing junctions. 183952 novel splicing junctions.
sampling 100% (244276143) splicing reads. 390637 splicing junctions. 206303 known splicing junctions. 184334 novel splicing junctions.