[W::hts_idx_load3] The index file is older than the data file: 1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16994721) splicing reads. 249950 splicing junctions. 178059 known splicing junctions. 71891 novel splicing junctions.
sampling 10% (33989442) splicing reads. 309305 splicing junctions. 191879 known splicing junctions. 117426 novel splicing junctions.
sampling 15% (50984164) splicing reads. 352946 splicing junctions. 199734 known splicing junctions. 153212 novel splicing junctions.
sampling 20% (67978885) splicing reads. 388313 splicing junctions. 205031 known splicing junctions. 183282 novel splicing junctions.
sampling 25% (84973607) splicing reads. 417842 splicing junctions. 209012 known splicing junctions. 208830 novel splicing junctions.
sampling 30% (101968328) splicing reads. 443641 splicing junctions. 212208 known splicing junctions. 231433 novel splicing junctions.
sampling 35% (118963049) splicing reads. 466049 splicing junctions. 214579 known splicing junctions. 251470 novel splicing junctions.
sampling 40% (135957771) splicing reads. 485733 splicing junctions. 216667 known splicing junctions. 269066 novel splicing junctions.
sampling 45% (152952492) splicing reads. 503390 splicing junctions. 218335 known splicing junctions. 285055 novel splicing junctions.
sampling 50% (169947214) splicing reads. 519047 splicing junctions. 219786 known splicing junctions. 299261 novel splicing junctions.
sampling 55% (186941935) splicing reads. 532867 splicing junctions. 220987 known splicing junctions. 311880 novel splicing junctions.
sampling 60% (203936656) splicing reads. 545093 splicing junctions. 221988 known splicing junctions. 323105 novel splicing junctions.
sampling 65% (220931378) splicing reads. 556206 splicing junctions. 222983 known splicing junctions. 333223 novel splicing junctions.
sampling 70% (237926099) splicing reads. 565953 splicing junctions. 223750 known splicing junctions. 342203 novel splicing junctions.
sampling 75% (254920821) splicing reads. 574502 splicing junctions. 224419 known splicing junctions. 350083 novel splicing junctions.
sampling 80% (271915542) splicing reads. 581851 splicing junctions. 225018 known splicing junctions. 356833 novel splicing junctions.
sampling 85% (288910263) splicing reads. 588198 splicing junctions. 225507 known splicing junctions. 362691 novel splicing junctions.
sampling 90% (305904985) splicing reads. 593570 splicing junctions. 225930 known splicing junctions. 367640 novel splicing junctions.
sampling 95% (322899706) splicing reads. 598050 splicing junctions. 226274 known splicing junctions. 371776 novel splicing junctions.
sampling 100% (339894428) splicing reads. 601305 splicing junctions. 226554 known splicing junctions. 374751 novel splicing junctions.