[W::hts_idx_load3] The index file is older than the data file: 1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11563445) splicing reads. 223910 splicing junctions. 175494 known splicing junctions. 48416 novel splicing junctions.
sampling 10% (23126891) splicing reads. 267017 splicing junctions. 188474 known splicing junctions. 78543 novel splicing junctions.
sampling 15% (34690337) splicing reads. 297692 splicing junctions. 195691 known splicing junctions. 102001 novel splicing junctions.
sampling 20% (46253783) splicing reads. 321810 splicing junctions. 200562 known splicing junctions. 121248 novel splicing junctions.
sampling 25% (57817229) splicing reads. 341776 splicing junctions. 204163 known splicing junctions. 137613 novel splicing junctions.
sampling 30% (69380675) splicing reads. 358893 splicing junctions. 206890 known splicing junctions. 152003 novel splicing junctions.
sampling 35% (80944121) splicing reads. 373585 splicing junctions. 209201 known splicing junctions. 164384 novel splicing junctions.
sampling 40% (92507567) splicing reads. 386513 splicing junctions. 211010 known splicing junctions. 175503 novel splicing junctions.
sampling 45% (104071013) splicing reads. 397586 splicing junctions. 212513 known splicing junctions. 185073 novel splicing junctions.
sampling 50% (115634459) splicing reads. 407236 splicing junctions. 213783 known splicing junctions. 193453 novel splicing junctions.
sampling 55% (127197905) splicing reads. 415812 splicing junctions. 214889 known splicing junctions. 200923 novel splicing junctions.
sampling 60% (138761351) splicing reads. 423372 splicing junctions. 215793 known splicing junctions. 207579 novel splicing junctions.
sampling 65% (150324797) splicing reads. 429881 splicing junctions. 216551 known splicing junctions. 213330 novel splicing junctions.
sampling 70% (161888243) splicing reads. 435618 splicing junctions. 217231 known splicing junctions. 218387 novel splicing junctions.
sampling 75% (173451689) splicing reads. 440461 splicing junctions. 217815 known splicing junctions. 222646 novel splicing junctions.
sampling 80% (185015135) splicing reads. 444511 splicing junctions. 218319 known splicing junctions. 226192 novel splicing junctions.
sampling 85% (196578581) splicing reads. 447861 splicing junctions. 218683 known splicing junctions. 229178 novel splicing junctions.
sampling 90% (208142027) splicing reads. 450438 splicing junctions. 219004 known splicing junctions. 231434 novel splicing junctions.
sampling 95% (219705473) splicing reads. 452439 splicing junctions. 219271 known splicing junctions. 233168 novel splicing junctions.
sampling 100% (231268919) splicing reads. 453662 splicing junctions. 219432 known splicing junctions. 234230 novel splicing junctions.