File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/5020220f0939ad6a1223160b5ce8db/.command.log
Size
49.2 KB
Attempt
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/60446498b55aa6efc029283a184293/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/5020220f0939ad6a1223160b5ce8db/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a6/4a4bf2afe7de789ec1bf0906becb61/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/60446498b55aa6efc029283a184293/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.SJ.out.tab
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/5020220f0939ad6a1223160b5ce8db/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/60446498b55aa6efc029283a184293/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Chimeric.out.junction
==> STAGING COMPLETE (7 inputs)

08:41:01 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes

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[13660000], rate=24105882.35/min 08:41:43 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 3557 cancer introns
08:41:44 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 
08:41:44 : INFO : Running: touch /tmp/nxf.WkK3JRR1ir/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok
08:41:44 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5  > 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 
08:41:44 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5  > 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 
08:41:44 : INFO : Running: touch /tmp/nxf.WkK3JRR1ir/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok
08:41:44 : INFO : -found 1622 cancer introns
08:41:44 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns  --cancer_introns 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 
08:41:44 : INFO :  Creating the BED File.
08:41:48 : INFO : Saving Bed File as 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed
08:41:48 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns  --cancer_introns 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 
08:41:48 : INFO : Running: touch /tmp/nxf.WkK3JRR1ir/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok
08:41:48 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed  --bam 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam  --output_prefix 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2 
[W::hts_idx_load3] The index file is older than the data file: 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
08:53:03 : INFO : Execution Time = 11.26 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed  --bam 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam  --output_prefix 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2 
08:53:03 : INFO : Running: touch /tmp/nxf.WkK3JRR1ir/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok
08:53:03 : INFO : Running: samtools sort -o 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam
08:53:04 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam
08:53:04 : INFO : Running: touch /tmp/nxf.WkK3JRR1ir/1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok
08:53:04 : INFO : Running: samtools sort -o 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam
[bam_sort_core] merging from 68 files and 1 in-memory blocks...