File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/87/1f76b9a8b7f6c466659542da3b2914/.command.err
Size
5.9 KB
Attempt
Done.
-parsing GTF file: /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.gtf
* Running CMD: /usr/local/bin/STAR  --runThreadN 8  --genomeDir /tmp/nxf.7wtUJyyAZ2/ctat_genome_lib_build_dir/ref_genome.fa.star.idx  --outSAMtype BAM SortedByCoordinate  --twopassMode None  --alignSJDBoverhangMin 10  --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705  --alignInsertionFlush Right   --alignMatesGapMax 100000  --alignIntronMax 100000  --outSAMattributes All  --readFilesIn /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_2.fastp.fastq.gz  --genomeFastaFiles /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.fa  --outSAMfilter KeepAllAddedReferences  --sjdbGTFfile /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.gtf  --alignSJstitchMismatchNmax 5 -1 5 5  --scoreGapNoncan -6  --readFilesCommand 'gunzip -c' 
* Running CMD: mv Aligned.sortedByCoord.out.bam 1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.star.sortedByCoord.out.bam
* Running CMD: samtools index 1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.star.sortedByCoord.out.bam
11:14:17 : INFO : Done.
-parsing GTF file: /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.gtf
-parsing /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam

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[80000]   -done parsing /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam.  Extracting junction info.
junction read elimination tally: $VAR1 = {
          'excessive soft clipping' => 549,
          ' ** passed ** ' => 747,
          'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 3,
          'exons hit < 2' => 3,
          'per_id < 96' => 353,
          'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 6
        };
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam.fusion_spanning_info.
 - counting read alignments among fusion contigs.

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[32000]   -fusion SPANNING read extraction for scaff: G2E3--TMEM260

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[24000]   -fusion SPANNING read extraction for scaff: TLK2--AC240565.1

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[6000]   -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1

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[22000]   -fusion SPANNING read extraction for scaff: TVP23C--CDRT4
-filtered reads reasons: $VAR1 = {
          'lacks exon overlap' => 466
        };
EM: Starting log likelihood: -634.498322
EM: Round [1] log likelihood: -634.498252
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam into /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords

CMD: touch /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords > /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.frag_coords > /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter  --genome_lib_dir ctat_genome_lib_build_dir  --max_promiscuity 10  --min_pct_dom_promiscuity 50 
CMD: cp /tmp/nxf.7wtUJyyAZ2/fi_workdir/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -613.860925
EM: Round [1] log likelihood: -613.860855
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz: 241747287
Warning - not locating file: /tmp/nxf.7wtUJyyAZ2/1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
ls: cannot access 'IGV_inputs': No such file or directory